摘要
目的:观察逆针"关元""三阴交"穴对去卵巢大鼠下丘脑-垂体-卵巢(HPO)轴的调节作用,探讨腧穴主治作用的特异性。方法:选取3月龄SD大鼠,设正常对照组(6只)、假手术组(6只)、模型组(6只)、逆针关元组(11只)、逆针三阴交组(11只)、逆针关元续针组(11只)、逆针三阴交续针组(11只),共7组。4组逆针组预先电针15次(隔日1次)后与模型组一同去除大鼠双侧卵巢造模。两组续针组于造模12d后继续电针5次(隔日1次)。采用酶联免疫法测定各组大鼠下丘脑中促性腺激素释放激素(GnRH),垂体中卵泡刺激素(FSH)、促黄体生成激素(LH)和子宫中雌二醇(E2)的含量。结果:与正常组相比,模型组大鼠E2水平显著降低(P<0.05),FSH、LH、GnRH水平明显升高(P<0.01);与模型组相比,各电针组E2水平均有不同程度升高,FSH、LH、GnRH水平均有不同程度降低(P<0.05,P<0.01);但各电针组间比较,差异无统计学意义(P>0.05)。结论:逆针"关元""三阴交"能够良性调节去卵巢大鼠紊乱的HPO轴功能,两穴之间未表现出明显穴位特异性。
Objective To observe the effect of preventive-electroacupuncture（PEA） at ＂Guanyuan＂（CV 4） and ＂San-yinjiao＂（SP 6）on hypothalamus-pituitary-ovary（HPO）activities in ovariectomized（OVX） rats,and to compare the specificity of efficacy of acupoints.Methods Sixty-two female SD rats were randomly divided into normal group（n=6）,sham-operation（sham） group（n=6）,model group（n=6）,PEA-Guanyuan（CV 4,PEA-CV 4） group（n=11）,PEA-Sanyinjiao（SP 6,PEA-SP 6） group（n=11）,complementary（C）-PEA-CV 4（n=11） and C-PEA-SP 6 group（n=11）.Climacterium model was duplicated by removing the bilateral ovaries.Before OVX,electroacupuncture（EA,2 Hz/100 Hz,） was applied to bilateral SP 6,CV 4 for 20 min,once every other day for 15 times.For rats of the C-PEA-CV 4 and C-PEA-SP 6 groups and 12 days after the OVX,PEA was applied to CV 4 and SP 6 again,once every other day for 5 times.For rats of the sham group,a little of fat nearby the ovary was removed.Enzyme-linked immunosorbent assay was used to measure the contents of gonadotropin releasing hormone（GnRH） in the hypothalamus,follicule-stimulating hormone（FSH） and luteinizing hormone（LH） in the hypophysis,and estrogen（E2） in the uterus.Results In comparison with the normal group,uterus E2 content was decreased significantly（P〈0.05）,and pituitary FSH and LH contents and hypothalamic GnRH content were increased obviously（P〈0.01） in the model group.Compared with the model group,uterus E2 levels in the PEA-CV 4 and C-PEA-CV 4 groups were increased apparently（P〈0.05）,while both pituitary FSH and hypothalamic GnRH levels in the PEA-CV 4,C-PEA-CV 4,PEA-SP 6 and C-PEA-SP 6 groups,and pituitary LH levels in the C-PEA-CV 4 and PEA-SP 6 groups were down-regulated markedly（P〈0.05,P〈0.01）,suggesting an improvement of the HPO axis activity following PEA and C-PEA.No significant differences were found between the normal and sham groups in uterus E2,pituitary FSH and LH,and hypothalamic GnRH contents,and between the model and PEA-SP 6 groups,and between the model and C-PEA-SP 6 groups in uterus E2 levels,and between the model and PEA-CV 4 groups,and between the model and C-PEA-SP 6 groups in pituitary LH levels（P〈0.05）.Conclusion Preventive EA of ＂Guanyuan＂（CV 4） and ＂Sanyinjiao＂（SP 6） can function in up-regulating uterus E2 level,and down-regulating pituitary FSH and LH contents and hypothalamic GnRH level in OVX rats,which may contribute to its efficacy in relieving climacteric syndrome in clinic.
出处
《针刺研究》
CAS
CSCD
北大核心
2012年第1期15-19,45,共6页
Acupuncture Research
基金
教育部新世纪优秀人才支持计划资助课题(No.0100413)
关键词
逆针
围绝经期综合征
去卵巢大鼠
下丘脑-垂体-卵巢轴
三阴交
关元
Preventive acupuncture
Climacteric syndrome
Ovariectomized rats
Hypothalamus-pituitary-ovary axis
Sanyinjiao（SP 6）
Guanyuan（CV 4）
