摘要
【目的】探讨玻璃体内注射 3 异丁基 1 甲基黄嘌呤 (IBMX)对切断视神经后视网膜节细胞存活的影响。【方法】用荧光素逆行示踪标记法和定量解剖学技术观察各组成年金黄地鼠于视神经切断 5、7、14d后视网膜节细胞的密度。【结果】①正常视网膜节细胞平均密度为 (2 0 0 7± 115 ) /mm2 ;②视神经切断 5、7、14d后 ,视网膜节细胞平均密度分别下降至 :(10 10± 131) /mm2 ,(782± 5 5 ) /mm2 和 (2 14± 30 ) /mm2 ;③给予DMSO/生理盐水的对照组在上述各时间段视网膜节细胞平均密度与单纯视神经切断组结果相似 ;④给予IBMX的实验组在 5、7、14d视网膜节细胞的平均密度分别为 (140 2± 6 9) /mm2 、(1182± 194) /mm2 和 (483± 17) /mm2 ,与视神经切断组和DMSO/生理盐水对照组相比在各时间段上均存在显著性差异 (P<0 0 5 )。【结论】IBMX可提高视神经切断后成年金黄地鼠视网膜节细胞的存活 ,IBMX可能通过提高胞内的cAMP水平而促进视神经切断后视网膜节细胞的存活。
Objective To investigate the effects of 3 isobutyl 1 methyl xanthine(IBMX) on survival of axotomized retinal ganglion cell (RGC) by intravitreal injection. Methods Fluorescent retrograde tracing method and quantitative anatomical techniques were used to measure the densities of RGCs in all groups of adult hamsters. Results ① In normal retinas,the mean densities of RGCs were (2 007±115)/mm 2. ② At 5,7,14 days after axotomy,the mean densities of RGCs decreased to (1 010±131)/mm 2,(782±55)/mm 2and(214±30)/mm 2,respectively. ③ In the control group treated with DMSO/sodium saline,the densities of RGCs at 5,7 and 14 days after axotomy were similar to those observed in the axotomized group. ④ In the group treated with IBMX,the densities of RGCs at 5,7 and 14 days after axotomy were (1 402±69)/mm 2,(1182±194)/mm 2 and (483±17)/mm 2,which were different from the axotomized group and DMSO/sodium saline controls( P <0 05). Conclusions IBMX improves RGCs survival after axotomy in adult hamsters,probably through elevating cellular cAMP level.
出处
《中山医科大学学报》
CSCD
2000年第2期96-99,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
国家自然科学基金!( 3 9870 2 66)
广东省自然科学基! ( 980 0 96)
关键词
视网膜节细胞
IBMX
细胞存活
药物作用
methy1 3 isobuty1 xanthine/pharmacology
retinal ganglion cells/drug effects
cell survival/drug effects
IBMX
