摘要
目的:探讨miR-101在宫颈癌组织中的表达及与宫颈癌临床病理特征的关系。方法:应用逆转录实时荧光定量聚合酶链反应(RT-PCR)检测40例宫颈癌组织miR-101水平,以30例子宫肌瘤患者作为正常对照,探讨其表达与宫颈癌临床病理特征的关系。结果:与正常宫颈组织比较,miR-101在宫颈癌组织中表达下调(0.54±0.32)倍,差异有统计学意义(P<0.05)。不同病理分级、间质浸润深度、淋巴结转移宫颈癌患者miR-101表达水平分别比较差异均具有统计学意义(P<0.05),而不同年龄、临床分期、肿瘤直径、绝经与否的宫颈癌患者miR-101表达水平分别比较差异均无统计学意义(P>0.05)。结论:miR-101的表达与宫颈癌的发生、转移和侵袭能力有关,可作为治疗及评价预后的参考指标,并可为肿瘤靶向治疗提供新靶点。
Objective： To explore the expression level of miR - 101 in cervical cancer tissue and its relationship with clinicopathological features of cervical cancer. Methods： RT - PCR was used to detect the expression level of miR - 101 in 40 cases with cervical cancer, 30 cases with hysteromyoma were selected as normal cantrol group, the relationship between miR - 10i expression and clinicopathological features of cervical cancer was analyzed. Results ： Compared with normal control group, the expression level of miR - 101 in cervical cancer group decreased by （0. 54 ±0. 32） times, there was significant difference （P 〈 0.05 ） . There was significant difference in the expression level of miR - 101 among the cases with cervical cancer of different pathological grades, depths of interstitial infiltration, and lymph node metastasis or not （P 〈 0. 05）, but there was no significant difference in the expression level of miR - 101 among the cases with different ages, clinical stages, diameters of tumor, menopause or not （P 〉 0. 05） . Conclusion： The expression of miR -101 is correlated with the occurrence, metastasis, and invasive ability of cervical cancer, and miR - 101 can be used as a reference index for treatment and predicting prognosis, which can provide a new target for targeted therapy of cervical cancer.
出处
《中国妇幼保健》
CAS
北大核心
2012年第7期1072-1074,共3页
Maternal and Child Health Care of China
基金
江苏省无锡市科技局科研项目〔CSE00949〕
南京医科大学科技发展基金面上项目〔08NMUM080〕
