摘要
目的:观察元胡止痛方肠吸收液对大鼠离体胸主动脉环张力的影响,探讨中药体外药理实验的新方法。方法:采用外翻肠囊法制备元胡止痛方0.25,0.5,1,2 h 4个时间点的肠吸收液;大鼠脱臼处死后迅速取出胸主动脉,用45 mmol.L-1KCl对大鼠胸主动脉环进行预收缩,采用累积加样法加入肠吸收液,累计依次加样至50,100,200,400,800,1 600μL,分别观察各组肠吸收液在不同加样量下对大鼠胸主动脉环张力的影响,每组重复观察6次。结果:2 h肠吸收液组随着加样量的增加血管舒张率逐渐增高,说明其血管舒张活性增强,并具有量-效关系;与空白组比较,累计加样至200μL时有显著性差异(P<0.05),累计加样至400,800,1 600μL时均有极显著性差异(P<0.01)。当累计加样量为1 600μL时,上述4组的血管舒张率均明显增高,与空白组比较,0.5 h组有显著性差异(P<0.05),1 h组和2 h组均有极显著性差异(P<0.01),结果提示随着吸收时间的延长,肠吸收液的血管舒张活性明显增强,呈现时-效关系。结论:元胡止痛方的肠吸收液具有显著的血管舒张活性,并存在量-效关系和时-效关系。
Objective：To observe in vitro effect of intestinal absorption solution of Yuanhu Zhitong preparation on the tension of rat thoracic aortic ring in order to provide new method for pharmacological experiments of traditional Chinese medicine in vitro.Method： Firstly,intestinal absorption solution of Yuanhu Zhitong preparation was prepared by everted gut sac method in vitro at 4 different time points（0.25,0.5,1,2 h）.The thoracic aortic was quickly removed after rats were sacrificed by dislocation.Then aortic rings were pre-contracted by KCl（45 mmol·L-1）,and the tension of thoracic aortic ring was observed by adding different volumes（50,100,200,400,800,1 600 μL） of the intestinal absorbed solution from Yuanhu Zhitong preparation,experiments were performed in six replicates（n=6 per group）.Result： The vasorelaxation rate was enhanced with the increased volumes of intestinal absorption solution at 2 h,the results indicated that the vasorelaxation was enhanced and a dose-effect relationship was found;Compared with control group of intestinal absorption solution without Yuanhu Zhitong prepation,when the volume of 200 μL was added,P0.05;when the volume of 400,800,1 600 μL was added,P0.01.When the volume of 1 600 μL was added,the vasorelaxation of 4 groups were all enhanced,compared with control group of intestinal absorption solution,ther was statistical significance in 0.5 h group,P0.05;1 h,2 h group significance at P0.01,the results indicated that the vasorelaxation was also enhanced with the delayed absorption time of Yuanhu Zhitong preparation and a time-effect relationship was observed.Conclusion： The results show that the intestinal absorption solution of Yuanhu Zhitong preparation may have significant vasorelaxation activity with a manner of dose-effect and time-effect relationship.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第5期117-120,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技支撑计划项目(2008BAI51B03)
中药质量控制技术国家工程实验室课题(655-30)
关键词
元胡止痛方
肠吸收液
胸主动脉环
活性
Yuanhu Zhitong preparation
intestinal absorption solution
aortic ring
activity
