摘要
目的:通过研究TNF-α和IL-13基因的多态性为哮喘的诊断提供基础。方法:选择105例哮喘患儿和正常儿童80例。采集外周静脉血,采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测TNF-α和IL-13基因多态性。结果:病例组等位基因TNF-αA的分布频率均显著高于对照组(P<0.05)。IL-13位点病例组等位基因A的分布频率远高于对照组(P<0.05)。结论:TNF-α和IL-13多态性可能做为确定哮喘易感人群的一个重要指标。
Objective： It was to provide a basis for asthma diagnosis through gene polymorphism of TNF- α IL- 13. Methods：Governed by the principles of voluntariness and cooperation, 105 patients with asthma and 80 controls were recruited. Blood sample from each one was analyzed using the polymerase chain reaction restriction fragment length polymorphisms（PCR- RFLP）technique for gene polymorphisms of TNF - α and IL - β. Results：The distribution rate of TNF- apolymorphism in patients group was higher than the control group（P 〈 0.05）. Meanwhile, the distribution rate of IL- β polymorphism in patients group was higher than the control group（P 〈 0.05）. Conclusion： TNF- α and IL- β polymorphism may identify asthma as an important indicator of vulnerable populations.
出处
《内蒙古医学杂志》
2011年第11期1296-1298,共3页
Inner Mongolia Medical Journal
