摘要
MYB蛋白是植物体中一类重要的转录因子,广泛参与植物生理代谢和发育过程的调节。本研究对利用RACE-PCR分离克隆的MYB转录因子基因GmMYBJ7的功能进行了初步研究。Norhern杂交对GmMYBJ7在不同组织中的表达情况进行了检测,结果只在茎、叶中检测到了GmMYBJ7的表达;酵母表达结果显示,GmMYBJ7具有明显的转录激活功能,β-半乳糖苷酶活性为24.31 U;半定量RT-PCR检测显示,在表达GmMYBJ7的转化烟草中,类黄酮代谢途径中的苯丙氨酸氨基裂解酶(PAL)、肉桂酸-4-羟化酶(C4H)、4-香豆酰辅酶A连接酶(4CL)、查尔酮合成酶(CHS)、黄烷酮-3-羟化酶(F3H)等关键酶的表达显著降低,结果表明,GmMYBJ7可能参与植物类黄酮合成的调控。
The MYB proteins play an important role in plant,which involved in the regulation of the process of metabolism and development extensively. The function of MYB transcription factor gene GmMYBJ7,which was isola- ted from soybean using the method of RACE-PCR, was to be clarified preliminarily in this paper. The expression pattern of GmMYBJ7 in different organs was studied using Northen blot. The results showed that the expression of GmMYBJ7 was detected in the leaves and stems. The transcriptional activation ability of GmMYBJ7 protein was con- firmed by the yeast system with β-galactosidase activity of 24.31 U. Semi-quantitative RT-PCR analysis indicated that GmMYBJ7 may also repress the expression of some flavonoid biosynthetic genes, such as PAL（ Phenylalanine ammonia lyase ） , C4H （ cinnamate-4-hydroxylase ） , 4CL （ 4-coumaroyl-CoA ligase ） , CHS （ Chalcone Synthase ） , CHI （chalcone isomerase）and F3 H （flavanone 3-hydroxylase） , which is considered to be related to flavonoid biosynthe- sis in palnt.
出处
《华北农学报》
CSCD
北大核心
2011年第5期107-111,共5页
Acta Agriculturae Boreali-Sinica
基金
中央级公益性科研院所科研业务费专项资金资助(2008-17)
关键词
大豆
MYB转录因子
基因表达调控
Soybean
MYB transcription factors
Gene expression and regulation
