摘要
半定量RT-PCR分析表明,水稻胚胎发育后期丰富蛋白基因OsLEA19a在水稻幼苗中的表达受干旱和高盐的诱导,说明OsLEA19a可能在水稻抗旱和抗盐中发挥作用。利用RT-PCR方法成功从水稻中克隆了OsLEA19a的cDNA。生物信息学分析表明,OsLEA19a基因编码一个由200个氨基酸残基组成的蛋白,蛋白分子量为20.48 kDa,等电点为5.89。OsLEA19a蛋白的5～48位氨基酸残基形成LEA_4结构域。OsLEA19a蛋白的二级结构有3个α-螺旋构象区域,没有伸展的β-片层构象,为亲水蛋白。进化树分析表明,OsLEA19a与单子叶植物第3组LEA蛋白的亲缘关系较近,而与双子叶植物的较远,OsLEA19a与单子叶植物第3组LEA蛋白的氨基酸一致性为48%～57%。
Semi-quantitative RT-PCR analysis revealed that OsLEA19a,a late embryogenesis abundant（LEA）protein gene,was induced by water deficit and salt stress,which suggested a role for OsLEA19a protein in water deficit and salt stress protection.The cDNA sequence of OsLEA19a was cloned from rice by RT-PCR.Bioinformatics analysis showed that OsLEA19a encoded a protein of 200 amino acids with a calculated molecular mass of 20.48 kDa and a theoretical pI of 5.89.OsLEA19a protein contains a Pfam：LEA_4 domain architecture at position 5-48,three α-helical domains and without β-sheet domain.In silico predictions showed OsLEA19a protein was strongly hydrophilic.The phylogenetic relationship between related group 3 LEA proteins from different plants was analyzed,which showed that OsLEA19a was more closely related to group 3 LEA proteins from monocots than from dicots.The amino acid sequence of OsLEA19a shows 48%-57% sequence identity with other members of group 3 LEA proteins from monocots.
出处
《华北农学报》
CSCD
北大核心
2011年第6期73-78,共6页
Acta Agriculturae Boreali-Sinica
基金
重庆高校创新团队建设计划(201040)
重庆市教育委员会科学技术研究项目(KJ111108)
重庆三峡学院科学研究计划(11ZD-16)
