摘要
灰飞虱是水稻生产上的一种重要害虫。对灰飞虱为害后水稻叶片的DAB组织化学染色表明,抗虫品种比感虫品种较早产生虫害响应,在为害3 d时就检测到H2O2的积累,随着为害时间延长,抗虫品种细胞内的H2O2积累程度逐渐下降,直至恢复至为害初期水平,而感虫品种细胞内H2O2的积累呈不断上升趋势。通过扫描电镜发现,抗虫品种能分泌某种物质使灰飞虱取食造成的伤口愈合,对植株起到了保护作用,而感虫品种则无类似反应。利用电子显微镜对水稻叶片细胞内的超微结构进行观察表明,灰飞虱为害3 d后,抗虫品种叶绿体结构完整,基粒及片层结构完好,而感虫品种中叶绿体内部结构排列无序;为害6 d后,抗感品种叶绿体膜结构均溶解,但抗虫品种内部片层结构依然存在,排列整齐,而感虫品种内部结构松散,排列不整齐。
The small brown planthopper（SBPH）,Laodelphax striatellus Fallén（Homoptera：Delphacide）,is a serious pest in rice（Oryza sativa L.）.Diaminobenzidine（DAB） hydrogen peroxide（H2O2） staining of rice leaf infested by SBPH indicated pest response was earlier in resistant rice than that in the susceptible.Accumulation of H2O2 was detected in the resistant variety infested by SBPH for 3 days but not in the susceptible rice.The H2O2 content declined gradually to the initial level in resistant rice cells but tended to increase in the susceptible rice with the damage extended.Observation with scanning electron microscope（SEM） on epidermis of affected rice leaves indicated that wound healing likely caused by some substance secreted by rice was found in the resistant variety and then protected the infected seedling but not existed in the susceptible.Observation with（TEM） on ultrastructure of rice leaf cells showed the chloroplast structure was integrate,Whose grana thylakoid and lamellar structure were intact in resistant rice but disordered arrangement of the internal chloroplast structure appeared in the susceptible variety infested by SBPH for 3 days.Chloroplast membrane structure dissolved in both tested rice and was loose and disordered in susceptible rice but lamellar structure still existed and arranged orderly when the tested rice seedling was infested for 6 days.
出处
《华北农学报》
CSCD
北大核心
2011年第6期207-211,共5页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金(30971746)
转基因生物新品种培育科技重大专项(2009ZX08009-046B)
关键词
水稻
灰飞虱
活性氧
超微结构
Rice
Small brown planthopper
Reactive oxygen species
Ultrastructure
