摘要
为探讨汞化合物对脏器及生殖的毒性,采用卵母细胞体外培养、体外受精的方法研究了汞对小鼠脏器系数、卵母细胞成熟和受精能力的影响。结果表明,0.5和1.5mg/kgBW汞对小鼠的肝脏、肾脏有明显的毒性作用,1.5mg/kgBW组还对卵巢有明显的毒性作用,并能显著降低超排卵的卵母细胞数;汞对体内卵母细胞生发泡破裂没有影响,但可以抑制卵母细胞第一极体的释放,影响卵母细胞的存活率并降低体外受精(IVF)率。结果提示,氯化汞可以影响卵母细胞的成熟,明显破坏卵母细胞的体外受精能力,损伤或降低小鼠的生殖能力。
In this paper the effects of mercury chloride on the coefficient of visceral organ of mouse and the meiotic maturation and the fertilization ability of mouse oocyte were studied by in vitro culture and in vitro fertilization (IVF) of mouse oocytes,etc.The results showed that mercury chloride (0 5 and 1 5mg/kg BW) had obviously toxicity to the liver and the kidney of mouse, and the dose of 1 5mg/kg BW could damage the ovary function and reduce the number of superovulation oocytes.The results also showed that mercury chloride could inhibit the extruding of the first polar body and affect the quality and the viability of mouse oocyte and reduce the rate of IVF,but little impact on germinal vesicle breakdown (GVBD) of mouse oocyte in vivo.The results indicated that mercury chloride could affect the meiotic maturation of mouse oocyte,obviously block the IVF and injury or make a reduction of reproductive capacity of mouse.
出处
《卫生研究》
CAS
CSCD
北大核心
2000年第2期75-77,共3页
Journal of Hygiene Research
基金
江苏省自然科学基金! ( BK990 6 4 )
关键词
氯化汞
卵母细胞
生发泡破裂
生殖毒性
mercury chloride,oocyte,germinal vesicle breakdown,the first polar body
