摘要
目的探讨联合检测抗核抗体(ANA)和抗双链DNA抗体在狼疮性肾炎(LN)诊断中的作用。方法对406例系统性红斑狼疮(SLE)患者(其中LN122例)和120例健康体检者采用间接免疫荧光法测定ANA,应用欧蒙印迹法测定抗双链DNA抗体。结果 406例SLE患者,ANA阳性率平均为94.49%;与对照组比较差异有统计学意义(P<0.001)。ANA阳性者核型,LN组核均质型和核颗粒型占84.35%,不伴肾炎组占72.12%(P<0.05)。ANA滴度:LN组较不伴肾炎组,低滴度结果低,而高滴度结果高(P<0.05)。ANA阳性者检测抗双链DNA抗体,其阳性率为:LN组65.22%;不伴肾炎组51.67%(P<0.05)。抗双链DNA抗体滴度,低滴度结果LN组64.00%,不伴肾炎组81.30%;高滴度结果LN组36.00%,不伴肾炎组18.70%(P<0.05)。两组抗双链DNA抗体高滴度时,其相应的ANA滴度:LN组高滴度结果占绝大多数,不伴肾炎组结果分布较均匀,两组间差异有统计学意义(P<0.05)。结论联合检测ANA及抗双链DNA抗体,并综合分析判断,对提高LN的诊断率及疗效观察、判断预后等方面具有重要意义。
Objective To explore the diagnostic significance of the combined detection of antinuclear antibody（ANA） and anti-ds DNA antibody in patients with lupus nephritis（LN）.Methods ANA was detected by indirect immunity fluorescent method and anti-ds DNA antibody was detected by EUROIMMUN blotting test in 406 patients with systemic lupus erythematosus（SLE）,including 122 cases with LN, and 120 health controls.Results Among 406 SLE patients,the average positive rate of ANA was 94.49%, which was significantly different with that in healthy control group（P0.001）.According to the karyotype in patients positive with ANA,homogeneous and speckled were 84.35% in LN group, and 72.12% in SLE without nephritis group,and there was statistical difference （P〈0.05）.According to ANA titer, lower titer were lower and higher titer were higher in LN group than that in SLE without nephritis group（P〈0.05）.Among ANA positive patients of these two groups, the positive rate of anti-ds DNA antibody was 65.22%, which was higher than 51.67% in SLE without nephritis group（P〈0.05）.The titer of anti-ds DNA antibody was almost low in SLE without nephritis group, and the high titer rate of it in LN group was obviously higher than that in SLE without nephritis group （P〈0.05）.When anti-ds DNA antibody in these two groups was with higher titer, their ANA titer were almost higher in LN group than that in SLE without nephritis group（P〈0.05）.Conclusion Combined detection of ANA and anti-ds DNA antibody could be used for the differential diagnosis of LN and other types of SLE,and could be with clinical significance in prognosis and providing rational adjuvant-therapies for such patients.
出处
《国际检验医学杂志》
CAS
2012年第3期287-289,共3页
International Journal of Laboratory Medicine
关键词
红斑狼疮
系统性
狼疮肾炎
抗体
抗核
抗双链DNA抗体
滴度
lupus erythematosus systemic lupus nephritis antibodies antinuclear anti-ds DNA antibody titer
