摘要
利用植物组织培养技术建立细毛山药茎尖培养和离体再生体系,探索影响山药组培的因素。结果表明:6-BA 2.0mg/L+NAA 0.5 mg/L能很好的诱导出愈伤组织;将诱导出的愈伤组织接种在KT 2.5 mg/L+NAA 0.02 mg/L和KT 2.0mg/L NAA 0.03 mg/L上都可分化出4～5个芽,且接种在KT 2.0 mg/L+NAA 0.03 mg/L上分化出的芽可直接长成健壮的植株。KT 1.5 mg/L+NAA 0.02 mg/L培养基对腋芽有较好的增殖效果,增值系数可达到3.8。将芽接种到KT 2.0mg/L+IAA 0.2 mg/L培养基上可健壮成长,也可作为继代培养基。
The system of shoot tip culture and regeneration in vitro of yam were established by plant tissue culture,and its influenced factors were explored.The results showed that：the calli were induced in the media with 6-BA 2.0 mg/L＋NAA 0.5 mg/L.4-5 buds developed after the callus were transferred to the media with KT 2.5 mg/L＋NAA 0.02 mg/L and KT 2.0 mg/L NAA 0.03 mg/L,and the shootlets grew strongly in the latter media.The media with KT 1.5 mg/L＋NAA 0.02 mg/L showed good proliferation effect for leaf buds,and the proliferative ratio was to 3.8.The media with KT 2.0 mg/L＋ IAA 0.2 mg/L was suitable for subculture.
出处
《种子》
CSCD
北大核心
2012年第2期48-50,56,共4页
Seed
基金
山东省农业良种工程项目(项目编号:109001)
关键词
细毛山药
组织培养
再生体系
茎尖
fine-root yam
tissue culture
regeneration system
shoot tip
