摘要
目的探讨新的长链非编码RNA（1ncRNA）UC001kfo在肝癌中的表达及与肝癌侵袭、转移的关系。方法收集肝脏组织60例，分为肝硬化组、肝癌组、癌旁组、门静脉转移肝癌组。原位杂交检测UC001kfo和ACTA2（α-平滑肌肌动蛋白的基因）在肝癌组织的表达。结果UC001kfo在肝硬化组不表达或低表达，在其他3组均为阳性表达，以上4组的表达值分别为113．30±11．79、137．59±6．23、148．78±8．23、160．28±9．47，方差分析显示UC001lifo表达差异有统计学意义（P〈0．01），4组间的差异有统计学意义（P〈0．01），分别为肝硬化组〈癌旁组织〈肝癌组〈门脉癌栓组。ACTA2的表达分别为109．89±9．74、125．22±32．16、149．06±8．43、156．57±8．86，ACTA2表达差异有统计学意义（P〈0．01），4组间差异有统计学意义（P〈0．05），表达分别为肝硬化组〈癌旁组织〈肝癌组〈门脉癌栓组。结论UC001kfo和ACTA2在肝癌中表达明显增加，特别是门静脉癌栓组，UC001kfo可能通过调控ACTA2的表达促进肝癌的侵袭转移。
Objective Investigate the expression of a new long non-coding RNA （IncRNA） UC001 kfo in liver cancer and its relations to the invasion and metastasis of liver cancer. Methods Collect 10 cases of liver cirrhosis tissue samples, 25 cases of liver cancer tissue samples, 25 cases of samples of the tissues near the cancer and 10 cases of samples of the tissues of portal vein transferred liver cancer. The in-situ hybridization method is used to test the expression of UC001kfo and ACTA2 （gene of a-smooth mus- cle actin） in liver cancer tissues. Results Results of the in-situ hybridization indicate that UC001kfo is not expressed or expressed in low quantity in the liver cirrhosis group, but it is expressed positively in all the liver cancer tissues, tissues near the cancer and portal vein transferred liver cancer tissues. The expres- sion values of the above four groups are respectively 113.30 ± 11.79, 137. 59 ±6. 23, 148.78 ±8. 23 and 160. 28 ±9.47. Variance analysis shows that the differences of UC001kfo expression have statistically sig- nificance （ P 〈 0. 01 ）. The differences of four groups have statistically significance （ P 〈 0. 01 ）, which can be arrayed as： liver cirrhosis group 〈 tissues near the cancer 〈 liver cancer group 〈 portal vein cancer embolus group. The ACTA2 values of the above four groups are respectively 109. 89 ±9. 74, 125.22 ± 32. 16, 149.06 ± 8.43 and 156. 57 ± 8. 86. Variance analysis shows that the differences of ACTA2 expres- sion have statistically significance （ P 〈 0.01 ）. The differences of four groups have statistically significance （ P 〈 0. 05 ） which can be arrayed as ： liver cirrhosis group 〈 tissues near the eancer 〈 liver cancer group 〈 portal vein cancer embolus group. Conclusion Expression of Uc001kfo and ACTA2 are obviously in- creased in liver cancer, especially the portal vein liver cancer embolus group. UC001kfo may have partici- pated in the occurrence of liver cancer by regulating and controlling the expression of ACTA2 and is related to the invasion and metastasis of liver cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第3期539-540,共2页
Chinese Journal of Experimental Surgery
