摘要
目的 通过生物信息学方法寻找Trim30α的人同源基因,观察候选Trim蛋白对TAB2诱导的NF-κB荧光素酶报告基因活性的影响.方法 经MEGA4软件对具有RBCC和SPRY结构域的Trim分子行进化树分析,用DNAMAN 6.0软件对候选Trim与Trim30α进行氨基酸序列比对,比较Trim30α相毗邻基因在小鼠和人染色体上的排列分布规律.构建Trim22的真核表达载体,用双萤光素酶报告基因系统检测Trim22对TAB2诱导的NF- κB报告基因活化的影响.结果 Trim30α与人Trim5α.、Trim6、Trim22、Trim34α来自进化树的同一分支节点；Trim22、Trim5α与Trim30α的氨基酸序列同源性分别为42.51％、45.47％；Trim22可以抑制TAB2诱导的NF-κB报告基因的活化,而Trim5α无显著抑制作用.结论 Trim22对TAB2诱导的NF-κB报告基因活化有负向调节作用,可能与Trim30α具有相类似的生物学功能.
Objective To screen for human Trim30a homologous gene via bioinformatic approaches and to investigate the impact of Trim protein candidates on TAB2-induced NF-kB luciferase reporter gene activity.Methods Phylogenetic tree of Trim molecules containing RBCC and SPRY domains was analyzed using MEGA4 software.The amino acid sequences of Trim candidates and Trim30a were compared using DNAMAN 6.0 for further comparison on the sequence and distribution of Trim30a proximal genes in human and murine chromosomes.Trim22 eukaryotic expression vector was constructed,and the impact of Trim22 on TAB2- induced NF-kB reporter gene activity was determined using dual- luciferase reporter gene system.Results Phylogenetic tree analysis showed that Trim30a originated from the identical bifurcation point with human Trim5a,Trim6,Trim22 and Trim34a.The amino acid sequence of Trim22 and Trim5a exhibited homogeneity of 42.51% and 45.47% with that of Trim30a,respectively.Trim22,but not Trim5a,inhibited TAB2-induced NF-kB reporter gene activation.Conclusion Trim22 is involved in negative regulation of TAB2-induced NF-kB reporter gene activation and may share similar biological properties with Trim30a.
出处
《中华生物医学工程杂志》
CAS
2011年第5期397-402,共6页
Chinese Journal of Biomedical Engineering
基金
滨州医学院科研启动基金项目（BY2010KYQD07）
