摘要
目的 建立对16种常见凝固酶阴性葡萄球菌（CNS）的快速分子鉴定.方法 用gap基因对16种常见CNS进行扩增和测序,获得序列在GenBank进行比对,构建进化树进行同源分析,同时与16S rRNA基因比较.结果 16种常见CNS中,gap基因相似率介于39% ～ 98%,人葡萄球菌和与人葡萄球菌亚种相似率最高（98%）,腐生葡萄球菌与木糖葡萄球菌相似率最低（39％）,16SrRNA基因相似率介于96% ～ 99%,至少2种细菌99%以上相似率,最多4种葡萄球菌99%以上相似率；进化树同源分析显示,两种方法在检测缓慢葡萄球菌与松鼠葡萄球菌、产色葡萄球菌与中间葡萄球菌和人葡萄球菌与人葡萄球菌亚种时,都有很高的可信度（99%以上）,而对于其他10种细菌,gap基因同源分析有较少的不可靠信度（49%,56%）,16S rRNA基因同源分析有较多不可靠信度（43%、43%、50%、56%、63%、65%、76%）.结论 gap基因序列分析能够对16种常见CNS进行准确鉴定,同源分析可信度要明显优于16S rRNA基因.
Objective To establish the rapid molecular diagnosis of 16 common coagulase negative Staphylococcus（CNS）.Methods DNA sequencing of 16 CNS would be obtained with gap gene.After the alignment gap gene sequences which were available in the GenBank,the bacteria were identified with homological alignment and phylogenetic tree,and compared with the 16S rRNA gene.Results The sequence similarity of the gap sequences ranged from 39% to 98% in 16 CNS.There were the highest similarity （98%） between S.hominis and S.hominis subsp,and the lowest（39% ） between S.saprophyticus and S.xylosus.The sequence similarity of the 16S rRNA sequences ranged from 96 to 98%,at least two species of bacteria similar rate of 99% and the most four species similar rate of 99%.Phylogenetic homology analysis showed that it was a high confidence（99% ） in the detection ofS.xylosus and S.lentus,S.chromogenes and S.intermedius,S.hominis and S.hominis subsp,but for 10 other species of bacteria,gap homology analysis has less unreliable confidence（49%,56% ） and 16S rRNA has more unreliable confidence（43%,43%,50%,56%,63%,65%,76% ）.Conclusion Analysis of gap sequence could identify 16 CNS timely and accurately,with higher confidence than 16S rRNA.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2011年第12期1129-1132,共4页
Chinese Journal of Microbiology and Immunology
基金
首都医学发展科研基金资助（2009-1023）
