摘要
探讨Bcl-2和Bax在C57／BL6小鼠海马发育和老化过程中的表达变化。方法：取胚龄（E）18、20d和生后（P）1、3、5、7、14、21、28d以及2、3、6、15、18个月（M）的CA7／BL6小鼠海马，应用免疫组织化学、免疫印迹法及图像分析技术检测Bcl-2和Bax蛋白的表达。结果：E18d～P7d，海马CA区及齿状回Bcl-2和Bax阳性细胞逐渐密集。P14-P21d，CA区两种阳性细胞稀疏；齿状回Bcl-2阳性细胞逐渐稀疏，Bax阳性细胞P14d密集，P21d稀疏。P28d后均趋于稳定。图像分析显示，E18d-P21d，除12）（3区Bcl-2的平均光密度值无差异外，BcP2和Pax均表现为先增大后降低的趋势。免疫印迹法结果显示，Bcl-2和Bax表达E18d～P7d逐渐增多，P14~P21d减少；P28d后趋于稳定。Bcl-2／Bax在P1d降低，P3d～P5d增高，P7d～P21d降低，P28d后趋于稳定。结论：Bcl-2和Bax在小鼠海马发育早期表达较多，成年及老年期处于较低水平，表明它们可能参与了海马的塑形过程。
To observe the changes of expression of Bcl-2 and Bax protein in the development and aging of C57/ BL6 mouse hippocampus. Methods： Embyronic （E） 18, 20 day （d） and postnatal 1, 3, 5, 7, 14, 28 d and 2, 3, 6, 15, 18 month （M） mice were used in this study. Each group included 8 mice. Immunohistochemistry and immunoblot asssay were used to detect the expression of Bcl-2 and Bax protein in the mice hippocampus of different age. Results： From El6 d to P7 d, Bcl-2 and Bax immunoreactive cells increased in the hippocampus. On P14 d-P21 d, the two kinds of immunoreactive cells decreased, except the Bax positive cells in dentate gyrus which increased on P14 d while decreased on P21 d. Then this two kinds of cells remained unchanged after P28 d. Additionally, the average optical density of Bcl-2 and Bax on El8 d-P21 d in- creased firstly and then decreased, except the Bcl-2 in dentate gyrus area. Immunoblotting analysis showed that the expres- sion of Bcl-2 and Bax protein increased on E18 d-P7 d, decreased on P14 d-P21 d, and remained unchanged after P28 d. Bcl- 2/Bax decreased on P1 d, increased on P3 d-P5 d, decreased on P7 d-P21 d, and remained unchanged on P28 d. Conclusion： The expression of Bcl-2 and Bax is high in the early stage of hippocampus development and then low in adult and senectitude. It may be related with the formation of the hippocampus.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2012年第1期56-58,62,共4页
Chinese Journal of Anatomy
