摘要
背景:小肠黏膜下层已经被证实可以引导许多组织的特异性再生。目的:将骨髓间充质干细胞复合猪小肠黏膜下层构建组织工程皮肤并移植修复兔糖尿病皮肤缺损,观察其对糖尿病皮肤缺损的愈合影响和疗效。方法:制作兔糖尿病皮肤缺损,将骨髓间充质干细胞与冻干、灭菌、水合后的猪小肠黏膜下层膜复合制作组织工程皮肤并覆盖修复兔糖尿病皮肤缺损,并与小肠黏膜下层支架组和空白对照组做对照。结果与结论:造模后1,2,4周,组织工程皮肤组缺损皮肤修复面积百分比显著大于小肠黏膜下层支架及空白对照组(P<0.05)。缺损皮肤修复组织观察组织工程皮肤组组织生长明显快于小肠黏膜下层组与空白对照组。3组均未见明显皮肤附件结构。表明骨髓间充质干细胞复合猪小肠黏膜下层构建的组织工程皮肤修复兔糖尿病皮肤缺损好于单纯小肠黏膜下层膜,无明显瘢痕组织的生成,可作为支架材料用于皮肤缺损的修复。
BACKGROUND：Small intestinal submucosa（SIS） has been shown to guide the specific regeneration of many tissues.OBJECTIVE：To construct tissueengineered skin by bone marrow mesenchymal stem cells（BMSCs） combined with SIS to treat fullthickness skin defects in rabbits with diabetes mellitus,and to observe the healing condition and effects.METHODS：Fullthickness skin defect models were prepared in rabbits with diabetes mellitus.BMSCs with SIS were used to construct tissue-engineered skin for fullthickness skin defects in comparison with SIS scaffold and blank control group.RESULTS AND CONCLUSION：The repairing area in the combination group was larger than that of SIS scaffold and blank control groups（P 0.05）.The rate of skin growth in the combination group was also faster than that of the other two groups.There were no skin appendage structures in the three groups.It was indicated that the satisfactory effect was obtained by tissue engineered skin transplantation,and the tissue engineered skin could be used to treat the fullthickness skin defects following diabetes mellitus.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
2012年第3期443-448,共6页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
云南省科技厅-昆明医学院联合资金资助(2007C0002R)~~
