摘要
目的:建立间苯三酚分光光度法测定植物饮料中戊聚糖的分析方法。方法:样品中戊聚糖经热盐酸水解产生戊糖,用间苯三酚测定水解液中戊糖间接分析戊聚糖含量。结果:方法线性范围为40 mg/L～400 mg/L,线性方程为y=0.0017x+0.0043,r=0.9991,方法的检出限为4 mg/L,对含量为1.45%的液体戊聚糖饮料进行6次平行测定,RSD为2.03%。对含量为16.8%的固体戊聚糖粉进行6次平行测定,RSD为2.33%。结论:该法快速、准确、检测限低、精密度高,适用于植物饮料中的戊聚糖的检测。
Objective：To establish an analytical method for determination of pentosan in vegetable beverage by phloroglucinol spectrophotometry. Methods： Pentosan in vegetable beverage was hydrolyzed to pentose by heated hydrochloric acid, and then the content of pentosan was analyzed by phloroglucinal spectrophotometric method. Results： The linear range of this method was 40 mg/L -400 mg/L, and the linear equation was y =0.0017x ＋0. 0043 with r =0.9991. The limit of detection of the method was 4 mg/L. The relative standard deviation for 6 parallel determinations was 2.03% when pentosan content was 1.45%. The relative standard deviation for 6 parallel determinations of pentosan powder with pentosan content of 16.8% was 2.33%. Conclusion ： This method is rapid, accurate, sensitive and low in determination limit, thus it was applicable in the detection of pentosan in vegetable beverage.
出处
《中国卫生检验杂志》
北大核心
2012年第2期227-228,230,共3页
Chinese Journal of Health Laboratory Technology
关键词
植物饮料
戊聚糖
间苯三酚
分光光度法
Vegetable beverage
Pentosan
Phloroglucinal
Spectrophotometric determination
