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蒙古绵羊原始生殖细胞(PGCs)的分离培养与鉴定

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摘要 本试验从绵羊胎儿的生殖嵴中分离出原始生殖细胞,经体外培养后进行了初步鉴定。为建立绵羊原始生殖细胞(primordial germ cells,PGCs)的培养体系,试验对比了不同培养条件对蒙古绵羊PGCs生长的影响,将得到的胚胎生殖细胞(EG细胞)作形态学、酶学和免疫荧光鉴定。结果表明:绵羊EG细胞集落隆起,边缘整齐,呈鸟巢状,细胞排列紧密;AKP染色为弱阳性;免疫荧光检测特异标志物Oct3/4呈弱阳性,SSEA-1、SSEA-3、SSEA-4、TRA-1-61、TRA-1-80呈强阳性。
作者 刘春霞 赵瑞媛 李少伟 周欢敏
机构地区 内蒙古农业大学生命科学学院 内蒙古医学院基础医学院
出处 《畜牧与兽医》 北大核心 2012年第2期54-57,共4页 Animal Husbandry & Veterinary Medicine
基金 国家863计划子课题"绵羊胚胎干细胞系的建立及诱导多能干细胞的研究"(2008AA101005)
关键词 绵羊 原始生殖细胞 培养 鉴定
分类号 S826.8 [农业科学—畜牧学]
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参考文献14

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畜牧与兽医
2012年 第2期

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