摘要
目的了解兰州地区腹泻患儿中杯状病毒和腺病毒感染的分子流行病学及临床特点。方法收集兰州大学第一医院2010年7月至2011年6月腹泻患儿粪便标本295份，采用RT-PCR或PCR的方法检测杯状病毒及腺病毒，腺病毒阳性标本利用多重PCR及巢式PCR的方法分型，并对序列进行分析。结果295份粪便标本中杯状病毒的检出率为13．2％（39／295），腺病毒的检出率是5．1％（15／295）。分型结果显示：杯状病毒中69．2％为诺如病毒，其余是札如病毒，诺如病毒中以GII-3（13例）为主，其次为GII-4（12例），GII-6（2例）；腺病毒主要以F组的41型（10／15）为主，同时还检测到1例A组的31型，2例B组的3型及C组的1例5型和1例6型，两种病毒均主要感染2岁以下儿童，无明显的季节高峰。结论杯状病毒和腺病毒是2010—2011年兰州地区病毒性腹泻患儿的重要病原，长期监测具有重要意义。
Objective To investigate the clinical and molecular epidemiology characteristics of calicivirus and adenovirus in children for viral diarrhea in Lanzhou. Methods Stool samples were collected from 295 children with diarrhea at the First Hospital of Lanzhou University, Gansu Province, China,between July 2010 and June 2011. Reverse transcription-polymerase chain reaction （RT-PCR） or PCR were used to detected calicivirus and adenovirus. The adenovirus positive samples were typed by nested PCR and multiple PCR. Results Of the 295 specimens, 13.2% （ 39/295 ） were positive for calicivirus, and 5.1% （ 15/295 ） were adenovirus. Typing and Phylogenetic analysis revealed that novirus GII-3 and adenovirus 41 were the dominant strains. Both calicivirus and adenovirus predominately affect children under the age of 2. In seasonal distribution,there was no obvious peak. Conclusion Human calicivirus and adenovirus are important pathogens of viral diarrhea, it is important to develop long-term systematic surveillance.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2012年第1期8-10,共3页
Chinese Journal of Experimental and Clinical Virology
