摘要
目的:研究氧化苦参碱(OMT)对大鼠急性心肌梗死诱发实验性心肌纤维化的保护作用及对TGF-β-Smads信号通路的影响。方法:采用冠状动脉结扎术复制大鼠急性心肌梗死模型,随机分为模型组、OMT高、中、低剂量组(50,25,12.5mg.kg-1)、卡托普利组(50 mg.kg-1);假手术组(Sham)只做冠状动脉穿线而不结扎。模型复制8周后采用HE与Masson染色分析心肌纤维化病变程度,半定量RT-PCR分析TGF-β-Smads信号系统mRNA的表达。结果:模型组急性心肌梗死8周后,病理组织学检查提示心肌细胞减少,细胞外基质沉积,胶原含量增加;RT-PCR结果提示TGF-β1,TβR1,Smad2,Smad3,Smad4 mRNA表达明显增加,而Smad7 mRNA的表达显著降低,与Sham组比较,差异显著。OMT显著抑制急性心肌梗死8周后诱发的实验性心肌纤维化,抑制TGF-β1,TβR1,Smad2,Smad3,Smad4 mRNA表达的上调和增加Smad7 mRNA的表达。结论:OMT对急性心肌梗死诱发实验性心肌纤维化具有一定的抑制作用,其作用机制与TGF-β-Smads信号系统密切相关。
Objective： To study the protective effect of oxymatrine（OMT） on myocardial fibrosis induced by acute myocardial infarction in rats and its effect on TGF-β-Smads signal pathway.Method： Arteria coronaria ligation-induced acute myocardial infarction model was established in rats.The survived rats were randomly allotted into the model group,50,25,12.5 mg·kg-1 OMT groups,the 50 mg·kg-1 captopril group,and the Sham-operated group which was treated as the model group without the arteria coranaria ligation.After 8 weeks of ligation,myocardial fibrosis was detected by HE and Masson staining,and the RT-PCR method were used to detect the expression of mRNA of TGF-β-Smads signal system.Result： The histopathological examination showed decrease in cardiocytes,deposition of extra-cellular matrix,and increase of collagen contents after 8 weeks of ligation.RT-PCR results showed that mRNA expressions of TGF-β1,TβR1,Smad2,Smad3 and Smad4 significantly increased,but mRNA expression of Smad7 is remarkable lower than the sham-operated group.Treatment with OMT for 8 weeks could remarkably inhibit myocardial fibrosis,decrease mRNA expressions of TGF-β1,TβR1,Smad2,Smad3,and Smad4,and increase mRNA expressions of Smad7.Conclusion： OMT has the inhibitory effect on the experimental myocardial fibrosis induced by AMI in rats.Its mechanism may be closely related to TGF-β-Smads signal system.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第5期632-636,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30701024
81173586)
贵州省科技攻关项目(黔科合[2007]1035号)
贵州省国际科技合作基金(黔科合外G字[2009]700115号)
