摘要
目的:探讨电针"丰隆"穴治疗高脂血症的作用机制。方法:将40只健康SD大鼠随机分为正常对照组、高脂饲料组、高脂饲料治疗组、高脂+普通饲料组及高脂+普通饲料治疗组,每组8只。两治疗组电针"丰隆"穴,每日1次。治疗30d后,检测各组大鼠血脂水平,即总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量,实时定量聚合酶链反应(RT-PCR)及免疫印迹法(Western Blotting)检测各组大鼠肝脏组织中ATP结合盒转运子A1(ABCA1)、过氧化体增殖物激活型受体α(PPARα)、肝X受体α(LXR-α)和视黄酸X受体α(RXR-α)基因表达的变化。结果:高脂饲料组大鼠血浆TC、LDL-C较正常对照组明显上升(均P<0.01);高脂+普通饲料组大鼠血浆TC、LDL-C与高脂饲料组比较明显下降(均P<0.01),较正常对照组仍升高明显(均P<0.01);电针"丰隆"穴治疗后,大鼠血浆TC、LDL-C明显下降(均P<0.01);TG、HDL-C变化不明显(均P>0.05)。与正常对照组比较,高脂饲料组大鼠肝脏ABCA1、PPARα、LXR-α及RXR-α的mRNA和蛋白含量均显著降低(均P<0.01);高脂+普通饲料组大鼠肝脏ABCA1、PPARα、LXR-α及RXR-α的mRNA和蛋白含量与高脂饲料组比较有所上升,较正常对照组仍降低明显(均P<0.01);电针治疗后,大鼠肝脏组织中ABCA1、PPARα、LXR-α及RXR-α的mRNA和蛋白含量均显著升高(均P<0.05)。结论:电针"丰隆"穴能够明显下调高脂血症大鼠血脂中TC、LDL-C水平,上调肝脏ABCA1、PPARα、LXR-α、RXR-α的基因表达,从而促进由其介导的胆固醇逆转运,对高脂血症具有一定的治疗作用。
Objective To explore the mechanism of electroacupuncture（EA） at ＂Fenglong＂（ST 40） in hyper lipidemia（HLP） rats. Methods Forty heahh SI） rats were randomly divided into a normal control group （group A）, a high fat forage feed group （group B） and a high fat forage feed treatment group （group C）, a high fat forage＋normal forage feed group（group D） and a high fat forage-l-normal forage feed treatment group（group E）, eight rats in each group. EA was applied at ＂Fenglong＂ （ST 40） for the rats in group C and group E, once daily. After treatment of 30 days, blood lipid levels of rats, including total cholesterol（TC）, triglyceride（TG）, low-densitylipoprotein cholesterol（LDL-C）, high-density lipoprotein cholesterol（HDL-C） in plasma were tested. Real time polymerase chain reaction（RT-PCR） and Western Blotting were applied to detect the gene expression changes of the contents of ATP-binding cassette transporter A1 （ABCA1）, Peroxisome proliferator-activated receptor alpha （PI＇ARa）, liver X receptor alpha （LXR a） and retinoid X receptor alpha（RXR-a） in liver tissue of rats. Results Compared with group A, the contents of TC, I.DL-C were significantly elevated in group B and group D （all P〈0.01）; compared with group B, above indices were significantly decreased in group D （all P〈0.01）. After the treatment of EA at ＂Fenglong＂（ST 40）, the contents of TC, LDL-C were significantly decreased（all P〈0.01）. and the contents ofTG, HDL-Cdid not change obviously（all P〉0.05）. Compared with group A, the mRNA and protein contents of ABCA1, PPARu, LXR-a and RXR a were decreased obviously in group B and group D （all P〈0.01）. But compared with group B, the above indices were decreased in the group D. There were signficantly in- creasing in the protein content of ABCAl, PPARa, RXR-a and I.XR-a mRNA after the treatment of EA（all P〈 0.05）. Cenclusien EA at ＂Fenglong＂ （ST 40） has some therapeutic effect on decrease the content of TC、LDL-C in rats of hyperlipemia and improve the gene expression of ABCAl, PPARa, LXR-a and RXR-a mRNA so as to promote reverse cholesterol transport.
出处
《中国针灸》
CAS
CSCD
北大核心
2012年第3期241-245,共5页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金项目:30873309
