期刊文献+

淫羊藿总黄酮对心力衰竭大鼠心肌细胞凋亡的调控作用研究 被引量:8

Effects of Total Flavonoids of Epimedium on Cardiac Cellular Apoptosis in Rats with Isoproterenol-induced Congestive Heart Failure
下载PDF
导出
摘要 目的研究淫羊藿总黄酮对异丙肾上腺素(ISO)所致充血性心力衰竭大鼠心肌细胞凋亡的影响。方法取SD雄性大鼠,采用皮下注射ISO方法建立心力衰竭模型。将造模成功的动物随机分为模型组、美托洛尔组及淫羊藿总黄酮小、中、大剂量组,另取皮下注射生理盐水的正常大鼠作为正常组。观察各组动物左室质量指数,血流动力学变化,神经内分泌因子的变化及心肌细胞凋亡相关指标。结果与模型组比较,淫羊藿总黄酮可呈剂量依赖性逆转左心室质量指数和血流动力学参数,明显抑制心力衰竭大鼠血清肿瘤坏死因子-α、去甲肾上腺素、血管紧张素Ⅱ水平,减轻心肌细胞DNA片断化,减少Bax、Caspse-9和Caspase-3表达,增加Bcl-2表达。结论淫羊藿总黄酮与β受体阻滞剂美托洛尔均能抑制皮下注射ISO所致心力衰竭大鼠心肌细胞凋亡,改善心功能。 Objective:To investigate the anti-apoptotic effects of total flavonoids of epimedium(TFE) on isopro- terenol-induced congestive heart failure (CHF) in rats. Methods:CHF was induced in male Sprague-Dawley rats by receiving subcutaneous injection of isoproterenoL Rats with heart failure were randomly divided into the control group,the metoprolol group,the TFE-low-dose group,the TFE-middle-dose group and the TFE-high-dose group. Meanwhile, rats receiving injection of saline serve were the normal group. Some indices were observed in these groups including the left ventricular weight index (LVWI), the hemodynamic changes, the neuroendocrine parame- ters changes and the myocardial apoptosis indices. Results:Compared with the control group,TFE dose-depen- dently reversed the changes of LVWI and hemodynamic parameters. In addition,TFE significantly inhibited serum levels of TNF-α, NE, Ang l] in rats with CHF. DNA fragments were obviously observed in the control group. The protein expressions of Bax, Caspase-9 and Caspase-3 were decreased and Bcl-2 was increased significantly in the TFE group as compared with those of the control group. Conclusion:TFE as well as metoprolol can decrease my- ocardial cell apoptosis and ameliorates LV dysfunction in isoDroterenol-induced CHF in Rats.
出处 《中国中医急症》 2012年第3期392-393,396,共3页 Journal of Emergency in Traditional Chinese Medicine
基金 江苏省南京市科技局科技发展指导计划课题(2005指导0523)
关键词 心力衰竭 淫羊藿总黄酮 心肌细胞 凋亡 Heart failure Total flavonoids of epimedium Cardiomyocyte Apoptosis
  • 相关文献

参考文献10

  • 1Wencker D,Chandra M,Nguyen K,et al.A mechanistic role for cardiac myocyte apoptosis in heart failure[J].J Clin Invest,2003,111:1497-1504.
  • 2Crow MT,Mani K,Nam YJ,et al.The mitochondrial death pathwayand cardiac myocyte apoptosis[J].Circ Res,2004,95:957-970.
  • 3Gustafsson AB,Gottlieb RA.Bcl-2 family members and apoptosis,taken to heart[J].Am J Physiol Cell Physiol,2007,292:45-51.
  • 4许兰芝,陈维宁.淫羊藿总黄酮对肾上腺素能受体阻断作用的研究[J].中国药理学通报,1994,10(4):311-313. 被引量:64
  • 5Van Zwieten PA.Drug treatment of congestive heart failure[J].SeminCardiothorac Vasc Anesth,2003,7(1):13-17.
  • 6Teerlink JR,Pfeffer JM,Pfeffer MA.Progressive ventricular remodel-ing in response to diffuse isoproterenol-induced myocardial necrosis inrats[J].Circ Res,1994,75(1):105-113.
  • 7Gustafsson AB,Gottlieb RA.Bcl-2 family mem bers and apoptosis,taken to heart[J].Am J Physiol Cell Physiol,2007,292:45-51.
  • 8Leri A,Claudio PP,LI Q,et al.Streteh-mediate release of angiotensinⅡinduces myocyte apoptosis by activating P53 that enhances the localrennin-angiotensin system and decreases the Bcl-2-to-Bax Proteinratio in the cell[J].J Clin Invest,1998:101:1326-1342.
  • 9Kang PM,Izumo S.Apoptosis in heart:basic mechanisms and impli-cations in cardiovascular diseases[J].Trends Mol Med,2003,9(4):177-182.
  • 10Ceconi C,Boraso A,Mele D,et al.TNF-αin patients with congestiveheart failure[J].Basic Res Cardiol,2004,99(1):12.

二级参考文献5

  • 1许兰芝,陈维宁.淫羊藿总黄酮对肾上腺素β_1受体的特异性阻断作用[J].潍坊医学院学报,1995,17(2):113-115. 被引量:5
  • 2汪芳裕,王楠,赵德化,盛宝恒.淫羊藿总黄酮的药理作用[J]第四军医大学学报,1986(02).
  • 3高尔,吕欣然.值测定[J]昌潍医学院学报,1983(02).
  • 4陈淑梅,许兰芝,吕欣然.—受体特异性实验研究[J]昌潍医学院学报,1982(02).
  • 5陈淑梅,贡沁燕,杨藻宸.钙拮抗剂戊脉安对兔主动脉条收缩作用的影响[J]上海第一医学院学报,1982(03).

共引文献63

同被引文献155

引证文献8

二级引证文献50

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部