摘要
目的研究醒脑静中环烯醚萜苷类有效成分栀子苷和京尼平龙胆双糖苷大鼠鼻腔吸收特性及其相关性。方法以体积校正法,采用改良的大鼠在体鼻循环模型研究栀子提取物单用、与醒脑静中冰片、麝香分别配伍及醒脑静全方配伍后栀子苷及京尼平龙胆双糖苷在大鼠鼻腔的吸收。结果栀子提取物中环烯醚萜苷类代表成分栀子苷和京尼平龙胆双糖苷的大鼠鼻腔吸收符合一级速率过程,为被动吸收。艾片、合成冰片及醒脑静全方配伍可促进栀子苷及京尼平龙胆双糖苷大鼠鼻腔吸收,人工麝香无显著促进作用。在不同配伍条件下两种成分的鼻腔吸收系数相关性良好,线性回归方程为K京尼平龙胆双糖苷=0.792 1K栀子苷+0.236 7(r=0.976 0)。结论醒脑静组方配伍可显著提高栀子苷及京尼平龙胆双糖苷的鼻腔吸收,方中环烯醚萜苷类成分具有相似的鼻腔吸收特性。
Objective To study the characteristic and correlation in rat nasal absorption of active components in Xingnaojing,geniposide（GE） and genipin-1-β-gentiobioside（GB）.Methods By modified volume correction method,an in situ rat nasal perfusion model was used to study the nasal absorption of GE and GB in single gardenia extract or combined with other different components,such as compatibility of borneol and musk respectively in Xingnaojing,and Xingnaojing prescription as well.Results The nasal absorption of GE and GB in gardenia extract fitted the first-order process and could be considered as passive absorption based on concentration gradient.When gardenia extract combined with borneol（nature or synthesis） and in whole formula of Xingnaojing,the nasal absorption of GE and GB was increased,while synthetic musk had no significant effect.The correlation of nasal absorption coefficients between two components in defferent compatibility was good and the equation of linear regression was KGB= 0.792 1 KGE＋ 0.236 7（r = 0.976 0）.Conclusion The nasal absorption of GE and GB in Xingnaojing formula could be remarkably increased and the nasal absorption characteristic of iridoid in prescription is similar.
出处
《中草药》
CAS
CSCD
北大核心
2012年第3期471-475,共5页
Chinese Traditional and Herbal Drugs
基金
"重大新药创制"科技重大专项(2009ZX09502-008)
教育部博士点基金资助项目(20090013110007)
国家自然科学基金资助项目(81073057
81102816)
北京中医药大学自主选题项目(JYBZZ-JS021)
北京中医药大学创新团队发展计划资助项目(2011-CXTD-13)
关键词
醒脑静
栀子苷
京尼平龙胆双糖苷
大鼠在体鼻循环
鼻腔吸收特性
Xingnaojing
geniposide（GE）
genipin-1-β-gentiobioside（GB）
in situ rat nasal perfusion
nasal absorption
