摘要
目的:探讨精浆弹性蛋白酶与精液主要参数和指标的关系。方法:用酶联免疫吸附法(ELISA)检测精浆中的弹性蛋白酶,按照WHO人类精液实验室手册要求进行精液常规分析、精子形态分析,检测精子顶体酶活性、精浆抗体(AsAb)、解脲支原体等,分析弹性蛋白酶与男性不育相关因素的关系。结果:209例男性不育患者中,43例患者精浆弹性蛋白酶≥290ng/ml,设为炎症组;166例患者精浆弹性蛋白酶<290ng/ml,设为非炎症组。炎症组的精子密度、精子活动率、a+b级活力精子率、精子顶体酶阳性率均低于非炎症组(P<0.05);而精子畸形率、精浆抗体(AsAb)、解脲支原体阳性率均高于非炎症组(P<0.05)。两组的精液量、PH值和液化时间差异无统计学意义。结论:精浆弹性蛋白酶水平与精液质量有密切的关系,生殖道感染是导致男性不育的重要原因。
Objective：To study the relationship between seminal plasma elastase and semen major paramelers. Methods：The level of seminal plasma elastase was detected by ELISA（Enzyme-linked immunosorbent assay）. Semen major parameters＇, abnormal morphological sperm rate, sperm aerosin reaction, AsAb and UU infection were measured and anal3;zed in 209 infertile men according to WHO laboratory handbook about human semen. Results： 43 cases were men whose level of seminal plasma elastase was equal to or higher than 290 ng/ml in 209 male infer tility, set up into inflammation group. The others （〈290 ng/ml） were 166 cases, set up into non-inflammation group. Semen concentration, motility, a＋b grade motility, and sperm acrosin reaction rate in inflammation group were lower than in non-inflammation group（P〈0.05）. Higher abnormal morphological sperm, UU infection rate and AsAb positive rate were observed in inflammation group（P〈0.05）. Sperm volume, PH value and liquefying time were not significantly different between inflammation and non-inflammation group（P〉 0.05 ）. Conclusions： The level of seminal plasma elastase is associated with sperm quality, which can be considered as one of the impor rant reasons resulting in male infertility.
出处
《临床泌尿外科杂志》
2012年第3期188-189,192,共3页
Journal of Clinical Urology
基金
广东省科技计划项目(No:2010B031600081)
关键词
精浆弹性蛋白酶
精液参数
男性不育
seminal plasma elastase
semen parameter
male infertility
