摘要
噬菌体呈现技术近年来得到小断改进和完善。基于loxP—CYe系统的细胞内重组技术是最为重要的改进。从双载体系统到单载体系统，从大样本抽样测序到肉眼直观判断，它使大库容量天然抗体库的构建变得简单易行，并有利于高亲和力抗体的获取。现对其改进和完善过程进行综述。
Phage display technology is improved in recent years. LoxP-cre system based in vivo recombination is the most important one. From double recombination system to single recombination system, from large scale sample sequencing to visual judgement, in vivo recombination makes it feasible to construct large natural antibody library, and it is favorable to get high affinity antibody. This review introduces the important improvement of in vivo recombination syslem.
出处
《肿瘤研究与临床》
CAS
2012年第2期131-133,共3页
Cancer Research and Clinic
基金
陕西省科技计划（2007K09-06）
西京医院助推计划（XJZT10M06）
关键词
抗体
噬菌体
细胞内蓐组
Antibodies
Phages
In vivo recombination
