摘要
建立测定麦冬中甲基沿阶草酮甲的高效液相色谱法。色谱柱:Hypersil C18柱(150mm×4.6 mm,5μm);流动相:甲醇-水(76∶24);流速:1.0 mL.min-1;检测波长:296 nm。实验结果:甲基沿阶草酮甲在0.445～2.67μg范围内线性关系良好,回归方程Y=3 252 213X-40 271(r=0.999 9),平均回收率为99.54%,RSD=1.44%(n=6)。结果表明,所建立的高效液相色谱法简便、快速、重复性好,可用于麦冬的质量控制。
A HPLC method was used to determine the content of Methyl ophiopogonanone A in Radix Ophiopogonis. The analysis was carried out on Hypersil C18 column （150mm×4.6 mm,5μm）. The mobile phase was MeOH-water （76： 24） with flow-rate of 1.0 mL · min -1. The eluate was detected at 296 nm in room temperature. The method was simple and had a good linear relationship. The linear range of Methyl ophiopogonanone A was 0.445 ~ 2.67 μg （ r = 0.999 9 ）. The recovery was 99.54% （ RSD = 1.44% ）. Therefore, this method was simple, fast and could be controlled effectively in Radix Ophiopogonis.
出处
《重庆理工大学学报（自然科学）》
CAS
2012年第2期52-54,共3页
Journal of Chongqing University of Technology：Natural Science
基金
国家科技重大专项课题(2010ZX09401-306-2-23)
重庆市科委产业化项目(CSTC
2008AB5116)
