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COPD患者诱导痰中DCs及CCR6的变化及意义 被引量:1

Significance and Changes of DCs and CCR6 in the induced sputum of patients with chronic obstructive pulmonary disease
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摘要 目的检测慢性阻塞性肺疾病(COPD)患者诱导痰中DCs的变化及CCR6的含量,分析DCs及趋化因子受体6(Chemkine receptor 6,CCR6)在COPD发病机制中的作用。方法以COPD患者为研究对象,并以无COPD者健康人作为对照,采用沙丁胺醇加高渗盐水超声雾化吸入方法诱导排痰,以流式细胞技术(FCM)检测痰液中DCs水平,以ELISA检测CCR6的含量。同时,常规方法测定肺功能FEV1%及FEV1/FVC。结果DCs在COPD分期的GOLD I级、GOLDⅡ级以及GOLDⅢ~Ⅳ级均高于正常对照组和吸烟无COPD组,且有显著统计学意义(P<0.01)。CCR6在COPD分期的GOLD I级、GOLDⅡ级以及GOLDⅢ~Ⅳ级均高于正常对照组和吸烟无COPD组,且有显著统计学意义(P<0.01)。DCs与COPD患者FEVl%之间呈负相关关系(P<0.05)。CCR6与COPD患者FEV1%之间呈负相关关系(P<0.01)。结论COPD患者DCs及CCR6显著升高,且与病情的严重程度明显相关,提示DCs及CCR6可能参与了COPD的炎症反应过程。 Objective To investigate the function of DCs and Chemkine necepnor 6 (CCR6) in the pathogenesis of ohronic obstructive pulmonary disease (COPD) through the change of DCs and CCR6 in the induced sputum of patients with CPOD.Methods The cells count determined by DCs were analyzed with flow cytometry in the sputum collected from COPD groups,
出处 《贵州医药》 CAS 2012年第2期111-114,共4页 Guizhou Medical Journal
基金 贵州省教育厅省长基金项目[黔科合SY字(2009)3053号]
关键词 慢性阻塞性肺疾病 树突状细胞 CD40 CD86 CCR6 诱导痰 肺功能 Chronic obstructive pulmonary disease(COPD) Dendritic cells (DCs) CD40 CD85 CCR6 The induced sputum Lung function
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