摘要
目的研究溃疡性结肠炎患者肠黏膜中的凋亡调控蛋白Bcl-2/Bax表达状态及其与细胞凋亡的关系,探讨溃疡性结肠炎的发病机制。方法应用脱氧核糖核酸转移酶介导的缺口末端标记技术和免疫组化方法分析36例溃疡性结肠炎患者和25例正常对照肠黏膜细胞凋亡、凋亡调控蛋白Bcl-2/Bax的表达。结果溃疡性结肠炎组凋亡调控蛋白Bcl-2/Bax的表达指数显著高于正常对照组(P<0.05),凋亡调控蛋白Bcl-2/Bax的表达与凋亡指数呈正相关(r=0.709,P=0.000;r=0.589,P=0.000)。结论溃疡性结肠炎患者肠黏膜凋亡调控蛋白Bcl-2/Bax表达增加,诱导肠黏膜细胞凋亡。
Objective To investigate the expression of Bcl-2/Bax and its relation with cell apoptosis in colonic mucosa in patients with ulcerative colitis, and to explore the pathogenesis mechanism of ulcerative colitis. Methods The cell apoptosis and the expression of Bcl-2/Bax in colonic mucosa were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end-labelling technique and immunohistochemistry in 36 patients with ulcerative colitis and 25 healthy subjects. Results The expression index of Bcl-2/Bax in ulcerative colitis group was significantly higher than that in normal control group （ P 〈 0.05 ）. The expression of Bcl-2/Bax in ulcerative colitis group was positively correlated with apoptosis index （ r = O. 709, P 〈 0.01 ; r = 0. 589, P 〈 0. Ol ）. Conclusion The expression of Bcl-2/Bax in colonic mucosa of patients with ulcerative colitis is increased, which can induce cell anontosis in colonic mucosa.
出处
《河北医药》
CAS
2012年第5期655-657,共3页
Hebei Medical Journal
