摘要
目的探讨羟基磷灰石纳米粒子运载Star3 shRNA对鼠胶质瘤C6细胞的促凋亡作用，并探讨相关机制．方法羟基磷灰石纳米粒子包被的Stat3 shRNA转染到c6胶质瘤细胞内，MTT法检测细胞增殖情况，应用流式细胞术及吖啶橙染色观察细胞周期分布及凋亡情况，TUNEL染色观察细胞的凋亡及增殖情况．结果羟基磷灰石纳米粒子运载Star3 shRNA转染C6细胞后，呈时间依赖性抑制C6细胞生长和增殖．通过流式细胞术检测证实：该质粒可使细胞阻滞在细胞周期的GO／G1期，细胞凋亡率明显增加（P＜0．05），与对照组比较差异有统计学意义；吖啶橙及TUNEL染色发现其明显促进细胞凋亡（P＜0．05），与对照组比较差异有统计学意义；结论羟基磷灰石纳米粒子运载Stat3 shRNA体外可明显抑制胶质瘤C6细胞增殖，并促进其凋亡，是治疗胶质瘤较理想的方法．
Objective This study is aimed to determine the pro-apoptotic effect of Stat3 shRNA delivered by hydroxyapatite nanoparticles on glioblastoma C6 cells and explore the related mechanisms. Method Stat3 shRNA was delivered into rat C6 glioma cells by hydroxyapatite nanoparticles. Cell growth was determined by MTF assay,and cell cycle distribution and apoptosis by flow cytometric and ethidium bromide/acridine orange staining assays. Apoptosis and proliferation of the C6 cells were determined by TUNEL assay. Results After Stat3 shRNA was transfected into C6 glioma cells through the hydroxyapatite nanoparticle delivery approach, the growth and proliferation of the ceils were considerably inhibited in a time-dependent way, and the flow cytometry verified that the cells were arrested in G0/G1 stage of the cell cycle and the apoptosis was significantly increased comparing with those of the control group （ P 〈 0.05 ）. Ethidium bromide/acridine orange staining and TUNEL staining demonstrated that it also promoted C6 cells apoptosis obviously comparing with control group （P〈0.05）. Conclusion Stat3 shRNA delivered by hydroxyapatite nanoparticles in vitro could significantly inhibit the growth of rat C6 glioma cells and promote their apoptosis,which may be an ideal method for the treatment of glioma.
出处
《北华大学学报（自然科学版）》
CAS
2012年第1期53-57,共5页
Journal of Beihua University（Natural Science）
基金
吉林省教育厅科学技术研究项目(2011146)
吉林市科技局科研项目(20113313901032235)
