摘要
采用荧光分光光度法 ,在体内、体外研究了溴氰菊酯对大鼠脑组织中EROD( 7 ethoxyresorufinO deethylase)酶活性的影响。结果表明 :大鼠经溴氰菊酯 12 5mg·kg-1·d-1通过腹腔注射连续 5天后 ,溴氰菊酯明显抑制全脑和大脑皮层EROD酶活性 ,抑制率分别是 3 2 0 % ,3 7 0 % ;在体外 ,溴氰菊酯浓度在 2× 10 -8～ 2× 10 -4 mol/L范围内对EROD活性无明显影响 ;进一步用蛋白印迹技术检测CYP1A1/1A2蛋白表达的变化 ,结果表明 :溴氰菊酯在体内明显抑制上述各脑区中CYP1A1/1A2蛋白表达。综合上述两方面结果 ,提示溴氰菊酯在体内能明显抑制脑内EROD活性 ,其机理与抑制该酶蛋白合成有关。
The EROD activity in rat brain was determined by flurophotometry under the treatment of DM in vivo and vitro.The results showed:in vivo,DM could markedly inhibit EROD activity in rat brain microsome after rats had been received DM (12 5mg·kg -1 ·d -1 ,ip)for 5 days.The inhibitory rate in whole brain,cerebral cortex were 32 0% and 37 7% respectively.However,in vitro,under the concentration of 2×10 -8 ～2×10 -4 mol/L,there was no obvious change of EROD activity in rat brain.Moreover,Western blot analysis indicated that DM could significantly reduce the expression of CYP1A1/1A2 protein.So the mechanism by which DM inhibit EROD activity in rat brain was releated to the reduction of CYP1A1/1A2 protein expression by DM.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第2期117-117,共1页
Chinese Journal of Public Health
基金
国家自然科学基金
