摘要
为研究镉对睾丸的生化毒作用机理 ,应用氯化镉对成年雄性SD大鼠进行腹腔染毒 ,染毒剂量分别为 0 2、0 4和0 8mg/kg体重。连续染毒 7天后将动物处死 ,测定睾丸中各种酶的活性 ,同时进行睾丸精子头计数并计算每日精子生成量。结果显示 ,中剂量组碱性磷酸酶 (ALP)和乳酸脱氢酶同工酶 X(LDH X)活性即有显著降低 ,高剂量组降低更明显。而其它一些酶的活性如酸性磷酸酶 (ACP)、乳酸脱氢酶同工酶(LDH)、葡萄糖 6 磷酸脱氢酶 (G 6PD)、山梨醇脱氢酶 (SDH)等及睾丸精子头计数和每日精子生成量仅在高剂量组出现明显降低。因此镉的雄性生殖毒性机理与酶的活性间存在一定关系。ALP和LDH X可作为反映镉雄性生殖毒性较敏感的生化指标。
In order to study biochemical toxicity effects of cadmium on testis,different doses of cadmium chloride(0 2,0 4,0 8mg Cd/kg body weight)were administrated ip to the adult male Sprague Dawley rats.After 7 days,the activities of enzymes in testis and testicular sperm head counts and daily sperm production were observed.The result showed the activities of alkaline phosphatases(ALP) and lactate dehydrogenase isozyme X (LDH X) decreased in the middle and high dose groups singificantly.But activities of other enzymes such as acid phosphatases(ACP),lactate dehydrogenase isozyme(LDH),glucose 6 phosphate dehydrogenase(G 6 PDH),sorbitol deydrogenase(SDH),testicular sperm head counts and daily sperm production decreased in the high dose group only.These results suggest cadmium toxicity on male reproductive system is associated to activities of testicular enzymes.ALP and LDH X are sensitive biochemical endpoints reflecting cadmium toxicity on male reproductive system.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第2期119-120,共2页
Chinese Journal of Public Health
基金
国家自然科学基金
关键词
镉
碱性磷酸酶
睾丸
毒性
酶学研究
Cadmium Alkaline phosphatases(ALP) Lactate dehydrogenase isozyme X(LDH X) Testicular sperm head counts
