摘要
目的研究在体及离体条件下不同力学环境对碱性成纤维细胞生长因子(basic fibroblast growth factor,bF-GF)表达的影响,探索力学因素在准分子激光原位角膜磨镶术(laser assisted in situ keratomileusis,LASIK)术后角膜损伤修复中的作用。方法建立不同切削量的LASIK手术动物模型,使在体角膜处于不同力学环境中,并于LASIK术后1周和1月处死实验动物提取组织蛋白。此外,采用Flexcell 4 000细胞力学加载系统对原代提取的兔角膜成纤维细胞施加频率为0.1 Hz、拉伸幅度分别为5%、10%和15%的周期性机械拉伸,并于拉伸后6 h和24 h后取细胞培养液上清。采用ELISA方法检测bFGF含量。结果 LASIK术后1周,角膜基质床残余30%组与对照组相比,bFGF含量显著增高(P<0.05);术后1月回落至正常水平,各组之间无显著性差异。不同时间点同一手术方式之间比较发现,仅30%组1周和1月有显著差异(P<0.05)。体外周期性拉伸实验表明拉伸6 h后1,5%拉伸组bFGF含量较对照组显著增高(P<0.05)2,4 h后显著性降低(P<0.05)。结论力学因素参与了早期角膜组织及角膜成纤维细胞bFGF表达的调节,bFGF在LASIK术后角膜组织修复中发挥了一定作用。
Objective To investigate the effect of different mechanical environment（in vivo and in vitro） on expression of basic fibroblast growth factor（bFGF） and explore the role of mechanical stimulation in corneal tissue repair after laser assisted in situ keratomileusis（LASIK） surgery.Methods Animal models by LASIK surgery were established to keep the corneas under different mechanical environment.The experimental animals were killed at the first week or the first month after LASIK surgery to obtain the corneas.In addition,the primary corneal fibroblasts were subjected to cyclic mechanical stretch（0.1 Hz;5%,10%,15% stretch;6 h or 24 h） using Flexcell 4 000 tension system.Expression of bFGF was determined by ELISA method.Results At the first week after LASIK surgery,expression of bFGF was increased significantly in 30% group（residual stroma bed accounting for 30% of the whole cornea）,as compared with the control group（P0.05）,and then it was decreased to the normal level in all groups at the first month after LASIK surgery.Analysis on the same surgery method at different time showed that there were significant differences only in 30% group at the first week and month（P0.05）.Cyclic stretch experiment in vitro indicated that bFGF expression in 15% stretch group was significantly increased after 6 h than that in the control group（P0.05）,with a significant decrease after 24 h（P0.05）.Conclusions Mechanical stimulation can regulate bFGF expression of corneal tissues and corneal frbroblasts,and bFGF plays a positive role in the early corneal tissue repair after LASIK surgery.
出处
《医用生物力学》
EI
CAS
CSCD
北大核心
2012年第1期72-76,共5页
Journal of Medical Biomechanics
基金
国家自然科学基金资助项目(10872140
11032008)
山西省自然科学基金资助项目(2008012013-82
010021004-2)
