摘要
目的:研究Th1、Th2各细胞因子在不同临床特征的子宫内膜异位症(EMs)患者差异性表达。方法:收集120例拟诊为"EMs"的患者临床资料。患者均于卵泡期行腹腔镜手术探查,术后经病理证实EMs者87例,非EMs者33例,将EMs组和非EMs组患者分别以主要主诉及临床特征如不孕、痛经/慢性盆腔痛、月经异常、卵巢受累进行再分组,应用ELISA法检测患者腹腔液中IL-2、IL-4、IL-6、IL-10、TNF-α、IFN-γ的表达。结果:以"痛经/盆腔痛"为主要临床特征的EMs组与非EMs组患者腹腔液TNF-α表达有显著差异,显示其与EMs盆腔痛有相关性(P=0.00);以"不孕"为主要临床特征的EMs组与非EMs组患者腹腔液IL-2表达有统计学差异(P=0.03);在检查有盆腔包块或月经异常的患者,术中探查卵巢子宫内膜异位囊肿或明确卵巢受累者的腹腔液IL-10在EMs组与非EMs组表达有统计学差异(P=0.01)。结论:在EMs患者Th1/Th2漂移的免疫异常中,不同的细胞因子在发病和临床特征的形成中发挥作用。
Objective： To study different expressions of Th1 and Th2 cell factors in endometriosis（EMs） patients with different clinical features.Methods： A total of 120 patients who were hospitalized and intended to be diagnosed as EMs in clinic,were examined by laparoscopic surgery during follicular phrase.After operation,87 cases were confirmed as EMs by pathology and 33 cases were non-EMs.Two groups of patients were regrouped according to clinical features： infertility,dysmenorrhea/chronic pelvic pain,menoxenia/ovarian involvement.ELISA method was used to measure different expressions of IL-2,IL-4,IL-6,IL-10,TNF-a,IFN-g in peritoneal fluid.Results： There was a significant difference in the expression of TNF-a in peritoneal fluid between EMs and non-EMs groups with dysmenorrhea/chronic pelvic pain（P=0.00）.There was a significant difference in the expression of IL-2 in peritoneal fluid between EMs and non-EMs groups with infertility（P=0.03）.In patients with pelvic mass and menoxenia,there was a significant difference in expression of IL-10 in peritoneal fluid between group with intraoperative exploration of endometrial cyst and group with confirmation of ovarian involvement（P=0.01）.Conclusion： In the dysimmunity of Th1 and Th2 drifting,different cytokines play different key roles in pathogenesis and formation of clinical features.
出处
《生殖与避孕》
CAS
CSCD
2012年第3期175-178,174,共5页
Reproduction and Contraception
基金
宁夏自然科学基金项目(NZ10123)
宁夏银川市科技局攻关项目
宁夏医科大学附属医院面上项目
