摘要
目的:观察survivin特异性RNAi载体对胰腺癌PANC-1细胞中survivin mRNA和蛋白的表达的抑制作用。方法:将U6启动子驱动的survivin特异性RNAi质粒载体和阴性对照质粒分别转染PANC-1细胞,用RT-PCR和Western blot分别检测survivin mRNA和蛋白的表达。结果:转染survivin特异性RNAi载体24 h和48 h后,PANC-1细胞survivin mRNA表达抑制率分别为(52.67±3.51)%和(75.33±3.06)%,蛋白表达抑制率分别为(58.00±3.61)%和(76.67±4.73)%;与阴性对照组比较,差异均有统计学意义(均P<0.05)。结论:以U6为启动子的survivin的RNAi载体,能有效地抑制胰腺癌细胞株PANC-1中survivin的表达。
Objective： To observe the inhibitory effect of survivin specific RNA interference（RNAi） vectors on the survivin mRNA and protein expression of pancreatic cancer PANC-1 cells. Methods： The plasmid vectors encoding U6 promoter-driven siRNA against survivin and the negative control plasmid were transfected into the pancreatic carcinoma PANC-1 cells,respectively.The mRNA and protein expression of survivin were detected by RT-PCR and Western blot,respectively. Results： In the PANC-1 cells transfected with RNAi vectors against survivin,the 24 h and 48 h inhibitory ratios on survivin mRNA expression were（52.67± 3.51）% and（75.33±3.06）%,and those on protein expression were（58.00±3.61）% and（76.67±4.73）%,respectively.All the differences had statistical significance compared with the negative control cells（all P0.05）. Conclusion： The RNAi vectors with U6 promoter can effectively inhibit the survivin expression in pancreatic carcinoma PANC-1 cells.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2012年第3期317-321,共5页
China Journal of General Surgery
关键词
胰腺肿瘤
存活素
RNA干扰
Pancreatic Neoplasms
Survivin
RNA Interference
