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奶牛乳腺蛋白组分析中蛋白质分步提取方法的建立

Establishment of Sequential Extraction Method in the Proteome Analysis of Mammary Gland from Dairy Cows
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摘要 全息蛋白质样品提取是蛋白组研究的先决条件,采用3种溶解性质不同的裂解液分步提取奶牛乳腺蛋白组,分别进行双向聚丙烯酰胺凝胶电泳分离,PDQUest 7.4分析凝胶图谱,发现其与常规方法相比,具有蛋白质提取率高、双向电泳(2-DE)分辨率高等优点。 The total sample preparation is a prerequisite for proteomic analysis.A three-step sequential protein extraction method was applied in proteomics analysis：using three kinds of solutions with differential solubility to replace one kind of solution.Proteins were separated by IEF and SDS-PAGE.And the patterns were analyzed with PDQUest 7.4 software.The method improved the efficiency of extraction and separation of the proteins compared with method of one solution.
作者 秦芝蕾 左谦益 伏小平
机构地区 甘肃农业大学动物医学院 兰州生物制品研究所有限责任公司实验动物室
出处 《动物医学进展》 CSCD 北大核心 2012年第3期66-68,共3页 Progress In Veterinary Medicine
关键词 分步提取 双向电泳 蛋白组 sequential extraction 2-DE proteome
分类号 S854.43 [农业科学—临床兽医学]
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参考文献7

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二级参考文献8

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动物医学进展
2012年 第3期

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