摘要
为了筛选夫西地酸高产培养基配方,提高夫西地酸发酵水平,以F17D2744为试验菌株,通过均匀设计方法,对不同碳氮源及微量元素等的配比实验,确定了最佳发酵培养基配方。试验结果表明:以蔗糖4.3%、糊精2%、酵母粉1%、蛋白胨3%、生物氮素0.59%、MgSO40.2%,KH2PO40.15%为发酵培养基配方,最终发酵效价较初始配方的发酵效价提高了234%。
In order to select high yield culture medium formula of fusidic acid and improve the level of fusidic acid fermentation,to have ratio test for the different carbon and nitrogen sources and trace elements with F17D2744 as the test strain,by uniform design method.To determine the optimum formula of fermentation medium.The test results showed that with sucrose 4.3%,dextrin 2%,yeast extract 1%,peptone 3%,biological nitrogen 0.59%,MgSO4 0.2%,KH2PO4 0.15% formula of fermentation medium,comparing final fermentation potency with initial formulations,the fermentation potency was enhanced 234%.
出处
《河北化工》
2012年第3期24-27,共4页
Hebei Chemical Industry
关键词
夫西地酸
发酵
培养基优化
均匀设计
fusidic acid
fermentation
medium optimization
uniform design
