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Fluoro-ruby retrograde tracing and three-dimensional visualization of the corticospinal tract in the guinea pig

Fluoro-ruby retrograde tracing and three-dimensional visualization of the corticospinal tract in the guinea pig
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摘要 Fluoro-ruby was injected into the posterior funiculus of the spinal cord in the cervical (C5-T2) and lumbar (L3-6) segments of adult guinea pigs. The spinal cord was cut into serial frozen sections. The Fluoro-ruby labeling was clearly delineated from the surrounding structure. The labeling traversed the cervical, thoracic and lumbar segments, and was located on the ventral portion of the posterior funiculus on the injected side, proximal to the intermediate zone of the dorsal gray matter. The fluorescence area narrowed rostro-caudally. The spinal cord, spinal cord gray matter and corticospinal tract were reconstructed using 3D-DOCTOR 4.0 software, resulting in a robust three-dimensional profile. Using functionality provided by the reconstruction software, free multi-angle observation and sectioning could be conducted on the spinal cord and corticospinal tract. Our experimental findings indicate that the Fluoro-ruby retrograde fluorescent tracing technique can accurately display the anatomical location of corticospinal tract in the guinea pig and that three-dimensional reconstruction software can be used to provide a three-dimensional image of the corticospinal tract. Fluoro-ruby was injected into the posterior funiculus of the spinal cord in the cervical (C5-T2) and lumbar (L3-6) segments of adult guinea pigs. The spinal cord was cut into serial frozen sections. The Fluoro-ruby labeling was clearly delineated from the surrounding structure. The labeling traversed the cervical, thoracic and lumbar segments, and was located on the ventral portion of the posterior funiculus on the injected side, proximal to the intermediate zone of the dorsal gray matter. The fluorescence area narrowed rostro-caudally. The spinal cord, spinal cord gray matter and corticospinal tract were reconstructed using 3D-DOCTOR 4.0 software, resulting in a robust three-dimensional profile. Using functionality provided by the reconstruction software, free multi-angle observation and sectioning could be conducted on the spinal cord and corticospinal tract. Our experimental findings indicate that the Fluoro-ruby retrograde fluorescent tracing technique can accurately display the anatomical location of corticospinal tract in the guinea pig and that three-dimensional reconstruction software can be used to provide a three-dimensional image of the corticospinal tract.
出处 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第5期363-367,共5页 中国神经再生研究(英文版)
基金 supported by a grant from the Priority Academic Program Development of Jiangsu Higher Education Institutions
关键词 corticospinal tract Fluoro-ruby retrograde fluorescent tracing technique three-dimensional reconstruction guinea pig corticospinal tract Fluoro-ruby retrograde fluorescent tracing technique three-dimensional reconstruction guinea pig
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  • 1张宝琳,顾绍光.电子计算机立体重建展示和分析大鼠脑干核团[J].承德医学院学报,1995,12(3):199-201. 被引量:3
  • 2郑华,万瑾,郑瑾,韩晓洁,俞亦龄,肖虹蕾,佘振珏,周国民.双荧光逆向追踪法研究大鼠视网膜节细胞的中枢投射[J].解剖学杂志,2006,29(1):25-29. 被引量:6
  • 3Hofman MA,Fliers E,Goudsmit E,et al.Morphometric analysis of the suprachiasmatic and paraventricular nuclei in the human brain:Sex difference and age-dependent changes.J Anat,1988,160(5):127-129.
  • 4KiKinis R,Langham GP,Moriarty TM,et al.Computer assited interactive three-dimensional planning for neurosurgical procedures.Neurosurgery,1996,38(4):640-651.
  • 5Fleckeisen CE,Harrison RV,Mount RJ.Effects of total cochlear hair cell loss on integrity of cochlear nucleus.Acta Otolaryngol (Stockh),1991,489(Suppl):23.
  • 6Melor H,Parker JJ,The extended protein kinase C superfamily[J]. Biochem J,1998,332(2):281-292.
  • 7Brown I.T.J.Projections and tennination of the comicospinal tracl in rodents[J].Exp Brain Res,1971,13(4):432-450.
  • 8Martin W J,Lin H,Wang H,Inflammation induccd up-regulotion of protein kinuse C gamma immunoreactivity in rat spinat cool correlates with enharaced nocieeptive processing [J].Neurosoience,1999,88(4):1267-1274.
  • 9Tcrashima T,Anatomy,Devclopment and lesion-induced plasticity of rodent cortionspinal tract[J].J Neurosci,1995,22(2):139-161.
  • 10Bradbury EJ.Moon LDF,et al.Chondroitinase ABC promotes functional reeovery after spinal sord injury[J].Nature,2002,416(6881):636-640.

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