摘要
对丙酮丁醇梭菌发酵木薯产溶剂进行研究,分别考察了N源、木薯含量、酶处理条件和培养基pH对发酵产丁醇的影响。结果表明:最佳的产丁醇发酵培养基为木薯粉120 g/L,乙酸铵6 g/L;木薯粉先用高温淀粉酶按酶量20 U/g、90℃水解60 min,再糊化30 min;发酵初始pH为6.0,发酵96 h。在此条件下,5 L发酵罐中丁醇产量达到13.5 g/L,总溶剂达到22.8 g/L。
Butanol was produced by fermentation of cassava by Clostridium acetobutylicum. The effects of nitrogen sources, cassava content, or-amylase loading, and initial pH of medium were investigated. The optimal conditions were obtained as follows： fermentation medium composing of 120 g/L cassava and 6 g/L ammonium acetate; the cassava hydrolyzed at 90 ~C for 60 min with high-temperature amylase of 20 U/g and gelatinized for 30 rain; and initial fermentation pH 6. 0. When fermentation was carried out in 5-L fermentor under these conditions for 96 h, the concentrations of butanol and total solvents reached 13.5 g/L and 22.8 g/L, respectively.
出处
《生物加工过程》
CAS
CSCD
2012年第2期6-10,共5页
Chinese Journal of Bioprocess Engineering
基金
国家高技术研究发展计划(863计划)资助项目(2011CB710803)
关键词
丁醇
木薯
丙酮丁醇梭菌
发酵
butanol
cassava
Clostridium acetobutylicum
fermentation
