摘要
目的:建立淫羊藿自乳化软胶囊中4种淫羊藿黄酮类成分的含量测定方法。方法:采用反相高效液相色谱法,色谱柱为Agilent Eclipse XDB-C18柱(250 mm×4.6 mm,5μm);流动相为水-乙腈(71∶29),检测波长270 nm,流速1.0 mL.min-1,柱温30℃。结果:朝藿定A,B,C和淫羊藿苷分别在0.952～9.52μg.mL-1,0.992～9.92μg.mL-1,0.974～9.74μg.mL-1,4.96～49.6μg.mL-1浓度范围内与峰面积呈良好的线性关系,其线性回归方程分别为:C=0.044 8A+0.410 6(r=0.999 9);C=0.029 9A+0.270 4(r=0.999 9);C=0.040 8A+0.270 4(r=0.999 9);C=0.028 3A+0.276 6(r=0.999 9),加样回收率(n=9)分别为100.1%;99.3%;100.3%和99.7%。结论:该方法操作简便、快速,准确度高,重复性好,稳定可靠,可作为淫羊藿自乳化软胶囊质量控制的方法。
Objective： To develop a RP-HPLC method for determination of four flavonoids in self-emulsif- ying soft capsules of Epimedium brevicornum extracts. Methods： The determination was performed on an Eclipse XDB-C18 column （250 mm ×4.6 mm, 5 μm） at 30 ℃ and a flow rate of 1.0 mL-min-l. The mobile phase was wa- ter-acetonitrile （71 ： 29）. The detection wavelength was set at 270 nm. Results ： The linear correlation equations for epimedin A, B, C and ieraiin were C=0.0448A ＋0.4106 （r=0.9999）, C=0.0299A＋0.2704 （r=0.9999）, C = 0. 040 8A ＋ 0. 270 4 （r = 0. 999 9）, and C = 0. 028 3A ＋ 0. 276 6 （ r = 0. 999 9）, respectively. A good linearity was obtained over the ranges of 0. 952 - 9.52 μg.mL^-1, 0. 992 - 9.92 μg.mL^-1, 0. 974 - 9.74 μg- mL^-1, and 4.96 -49.6 μg.mL^-1 , respectively. The recoveries were 100.1% , 99.3% , 100.3% and 99.7%. Conclusion： The method is simple and suitable to control the quality of self-emulsifying soft capsules of Epimedium brevicornum extracts.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2012年第6期697-700,共4页
Chinese Journal of New Drugs
基金
吉林省教育厅科学技术基金(吉教科合字2010第342号)
通化师范学院自然科学基金(201061)
