摘要
目的建立腹腔注射穿孔性腹膜炎腹水(PPAF)诱导大鼠急性肺损伤(ALI)模型,探讨ALI的发生机理和L-精氨酸(L-Arg)的作用。方法 60只SD大鼠建立消化道穿孔性腹膜炎(PP)模型,收集PPAF;48只大鼠按随机数字表法分为NS组(n=16),PPAF组(n=16)和L-Arg组(n=16)。各组大鼠再按随机数字表法分为7 h和12 h 2个亚组。检测外周血WBC计数、血清NO及脂质过氧化物丙二醛(MDA)水平、肺组织病理损害评分、肺组织湿/干质量比、肺组织髓过氧化物酶(MPO)水平和肺细胞凋亡情况。结果 PPAF组各时间点血WBC计数、血清NO及MDA水平、肺组织MPO水平、肺组织病理损害评分、湿/干质量比和细胞凋亡率均高于NS组(P<0.01)。L-Arg组血清NO水平显著高于PPAF组(P<0.01),血清MDA水平、肺组织MPO水平、肺组织病理损害评分和湿/干质量比以及细胞凋亡率均显著低于PPAF组(P<0.05)。PPAF组和L-Arg组12 h血清NO水平、肺湿/干质量比和细胞凋亡率均显著高于7 h(P=0.000)。PPAF组和L-Arg组血清NO水平与血清MDA水平(r=-0.257,P=0.021)、肺组织MPO水平(r=-0.444,P=0.011)和肺细胞凋亡率(r=-0.351,P=0.010)呈负相关。各组血清MDA与肺细胞凋亡率呈正相关(r=0.969,P<0.001)。结论腹腔注射PPAF可诱导大鼠ALI,肺内氧化反应增强和细胞凋亡参与了ALI的发生,而L-Arg对ALI具有保护作用。
Objective To investigate the pathogenesis of acute lung injury in rats induced by intra-peritoneally injection of perforative peritonitis ascitic fluids（PPAF）and the role of L-arginine（L-Arg）in acute lung injury in this model.Methods Perforative peritonitis（PP）models were established in 60 rats and PPAF were collected.Forty-eight rats were randomly divided equally into NS group,PPAF group,and L-Arg group.Rats were randomly subjected to death at 7 h and 12 h.Peripheral blood WBC were counted,levels of NO and malondialdehyde（MDA）in serum were examined.Lung injury score and wet/dry ratio were evaluated,and level of myeloperoxidase（MPO）in lung tissues and lung cell apoptosis were tested.Results WBC count of peripheral blood,levels of NO and MDA in serum,level of MPO in lung tissue,lung injury score,wet/dry ratio,and lung cell apoptosis rate in PPAF group were significantly higher than that in NS group at each time point（P 0.01）.Level of NO in serum in L-Arg group was higher than that in PPAF group（P0.01）,but lower level of MDA in serum,lower level of MPO in lung tissue and lung injury score,lower wet/dry ratio,and lung cell apoptosis rate were observed in L-Arg group（P0.05）.In PPAF group and L-Arg group,level of NO in serum,wet/dry ratio,and lung cell apoptosis rate were higher at 12 h than that at 7 h（P = 0.000）.Serum NO level was in negative correlation with serum MDA level（r =-0.257,P=0.021）,MPO level in lung tissue（r=-0.444,P=0.011）,and lung cell apoptosis（r =-0.351,P =0.010）in PPAF group and L-Arg group,but serum MDA level was in positive correlation with cell apoptosis（r = 0.969,P 0.001）in each group.Conclusions Acute lung injury rats model can be established by intra-peritoneally injection of PPAF.Enhanced oxidizing reaction and cell apoptosis take part in the occurrence of acute lung injury.L-Arg plays a protective role in acute lung injury.
出处
《中国普外基础与临床杂志》
CAS
2012年第3期282-287,共6页
Chinese Journal of Bases and Clinics In General Surgery
关键词
腹膜炎
腹水
急性肺损伤
细胞凋亡
一氧化氮
Peritonitis
Ascitic fluids
Acute lung injury
Cell apoptosis
Nitric oxide
