摘要
近年公认肾细胞癌起源于近曲小管,作者应用4种肾小管抗原的单克隆抗体(mAb)对38例成人肾及32例肾细胞癌进行免疫组化研究;发现这些mAb在肾脏有稳定的染色类型,肾细胞癌多数表达近曲小管抗原,少数表达远曲小管、集合管抗原。经肿瘤细胞类型分析,透明细胞癌主要起源于近曲小管,颗粒细胞癌可能起源于远曲小管。
Although it is now generally accepted that renal cell carcinoma (RCC) arises from proximal convoluted tubules (PCT), there are some features of RCC difficult to interpret by the hypothesis. We employed four monoclonal antibodies (mAbs) against different tubules to determine the antigenic expression of adult kidney and RCC with the avidin-biotin peroxidase complex (ABC) technique.The results in 38 adult kidney showed: the mAb Uro-2 stained intensively the epithelial cell of PCT and weakly glomeruli; the mAb against Tamm-Horsfall glycoprotein reacted with the cellular membranes of ascending thick limb of Henle's loop and distal tubules (DCT); the mAb Uro-5 bound to the epithelial cells of DCT, collecting ducts (CD); the mAb Uro-10 outlined the PCT. On the basis of immunoanatomic study of normal kidney we used these mAbs to detect the antigens of 32 cases of RCC. 13 cases (40.6%) only expressed the antigns of PCT and indicated the origin of this site. Five cases (15.6%) presented exclusively the antigen of DCT and were considered arising from the cells of them. Two cases 'had the antigen of CD and suggested deriving from this segment of nephron.Analysing the data in the literature and our results we have come to a preliminary conclusion that (1) not all RCC originate from the PCT, besides this portion of the nephron RCC also derive from the other segments of renal tubules, including DCT and CD; (2) the staining patterns of the mAbs are typical and consistent, and useful in immuno-microanatomy of kidney.
出处
《北京医科大学学报》
CSCD
1989年第3期187-190,261,共4页
Journal of Peking University(Health Sciences)
基金
自然科学基金
关键词
肾细胞癌
细胞起源
单克隆抗体
Carcinoma, renal cell Cellular origin Antibody, monoclonal
