摘要
目的:比较不同提取方法对首乌藤多糖提取效率的影响。方法:采用苯酚-硫酸法测定多糖含量,以粗多糖得率和多糖质量分数为评价指标,对首乌藤多糖不同提取方法进行研究。结果:6种方法中以氢氧化钠提取方法的效果最优,粗多糖得率和多糖质量分数可达25.13%和21.46%。结论:氢氧化钠提取首乌藤多糖是一种效率高且稳定可行的提取方法。
Objective:To compare effect of different extraction methods to extraction efficiency of polysaccharide from Caulis Polygoni Multiflori.Method:The content of polysaccharide was determined by phenol-sulfuric acid method,study on different extraction methods for polysaccharide from Caulis Polygoni Multiflori with the content and yield of polysaccharide as indexes.Result:Sodiumhydroxide extraction method was the best by comparing with other five methods,yield and the content of polysaccharide were up to 25.13%,21.46%,respectively.Conclusion:Sodiumhydroxide extraction was proved to be a high efficiency and stable method for extraction of polysaccharide from Caulis Polygoni Multiflori.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期38-40,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
郑州市金水区科技攻关项目(20093534)
关键词
首乌藤多糖
水提
碱提
酶提
酶-水提
polysaccharide from Caulis Polygoni Multiflori
water extraction
alkali extraction
enzyme extraction
enzyme-water extraction