摘要
利用色氨酸残基作为内源荧光探针在膜模拟剂（各种表面活性剂）和不同变性剂中对大肠杆菌碱性磷酸酶（AP）的构象变化进行了系统的研究。通过测定在不同变性时间下盐酸肌浓度对荧光强度的影响以及荧光强度随pH有规律的变化，进一步证实了该蛋白质变性过程中形成较稳定中间态的结论。
The conformational change of Escherichia coli alkaline phosphatase (AP) was systematcally studied using tryptophan residues as an intrinsic fluorescent probe in various media, such as anionic type surfactant of sodium dodecyl sulfate(SDS), cationic type of cetyltrimethylammonium bromide (CTMAB),and nonionic type of polyoxyethylene (23 ) dodecanol (Brij-35 ), guanidine hydrochloride (Gdn· HCl),urea and acid. A regular change in fluorescence intensity at differen time could be determined due to the change of concentration of Cdn· HCl or low pH. The conclusion was further confirmed that the protein formed a stable intermediates state in the denatured processes.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2000年第1期57-60,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金!29575204
29875016
关键词
碱性磷酸酶
色氨酸残基
蛋白质构象
荧光探针
AP
Escherichia coli alkaline phosphatase, tryptophan residues,conformation of protein,fluorescent probe
