摘要
目的:参连汤及其有效组分配伍对α-葡萄糖苷酶抑制作用的比较。方法:制备参连汤的不同溶剂提取样品及不同比例有效组分配伍样品,应用以蔗糖为底物的α-葡萄糖苷酶-抑制剂-96微孔板筛选模型,考察它们对α-葡萄糖苷酶的影响。结果:各组样品均具有一定的α-葡萄糖苷酶抑制活性,其中有效组分黄连总生物碱、人参总皂苷8:2配伍时作用最佳(IC50=1.2209mg·mL-1)。结论:有效组分配伍时具有协同作用,并明显优于参连汤传统应用,为合理开发古方参连汤提供科学依据。
Objective：ShenLian decoction compared with its effective combination components on inhibition effect of α-glucosidase. Methods： Preparated the ShenLian decoction, its different solvent extraction and the compatibility proportion of active component, used sucrose as the substrate of α-glucosidase enzyme-inhibitor-96 microplate screening model investigated the influence of α-glucosidase. Results： Each part has the inhibitory activity of α-glucosidase. The best compatibility of effective component is 8：2 （IC50 = 1. 220 9 mg· mL^-1） between alkaloids of rhizoma coptidis and ginsenoside. Conclusion： The compatibility of the effective components of synergies is better than the traditional application ShenLian decoction significantly. The results provide scientific basis for the rational development of the ShenLian decoction.
出处
《中国现代中药》
CAS
2012年第3期22-24,共3页
Modern Chinese Medicine
基金
吉林省科技厅重大项目--人参黄连药对拮抗糖尿病活性部位的研究(20090934)
关键词
参连汤
黄连总生物碱
人参总皂苷
Α-葡萄糖苷酶
ShenLian decoction
Total alkaloids of rhizoma coptidis
Total ginsenoside
α-glucosidase
