摘要
目的了解Semaphorins-3A蛋白在生后发育期大鼠脑中的分布特征和表达变化。方法随机选择生后0d、7d、14d、21d和28d日龄SD大鼠，通过免疫组化法观察Semaphorins-3A阳性细胞在大脑皮层、海马、齿状回和内嗅皮层内的分布和表达量。结果出生后大鼠Semaphorins-3A阳性细胞广泛密集的分布于顶、枕、额、颞、岛叶、扣带皮质、梨状前皮质和杏仁周皮质中除分子层（Ⅰ）以外的颗粒细胞层（Ⅱ）、外椎体细胞层（Ⅲ）、内颗粒细胞层（Ⅳ）、内椎体细胞层（Ⅴ）和多行层（Ⅵ）。Semaphorins-3A蛋白在0d、7d、14d、21d和28d日龄大鼠的内嗅皮层中表达量分别为84916．23±3266．34、77711．41±2634．26、74124．25±3989．09、65887．63±3406．57和57705．96±3136．35（IOD），表达区域缩小在Ⅱ-Ⅵ层的部分层次。Semaphorins．3A阳性细胞也分布于各日龄大鼠的齿状回、CAl、CA3区颗粒细胞层，少数散在分布于多行层，齿状回、CAl、CA3区内Semaphorins-3A表达量在各日龄之间具有明显差异（P〈0．01）。结论Sema-phonns-3A阳性细胞广泛分布于发育期大鼠脑各脑叶及海马CAl、CA3、DG区内，并随年龄的增长分布范围逐渐缩小。
Objective To study the distribution and expression of Semaphorins-3A protein in brain of postnatal rats. Methods Semaphorins-3A positive cells were observed by immunohistochemistry in the cerebral cortex,hippocampus,dentate gyrus and entorhinal cortex in postnatal 0d,7d, 14d,21d and 28d of Sprague Dawley rats. Results Semaphorins-3A positive cells widely distributed in the granule cell layer （ Ⅱ ） , external pyramidal cell layer （ Ⅲ） , internal granular cell layer （Ⅳ ） , pyramidal cell layer （ Ⅴ ） and layer of polymorphous cells （ Ⅵ） , in addition to the molecular layer （Ⅰ ） of the parietal, occipital, frontal, temporal, insular, cingulate cortex, piriform cortex and entorhinal cortex with postnatal 0d,7d, 14d,21 d and 28d rats. The amount of semaphorins-3A positive cells（IOD） in the entorhinal cortex was 84916.23 ± 3266.34 in P0d, 77711. 41 ± 2634.26 in PTd, 74124.25 ± 3989.09 in P14d,65887.63 ＋ 3406.57 in P21d and 57705.96±3136.35 in P28d,meanwhile the re gion of semaphorins-3A positive cells narrowed in the part level with Ⅱ -Ⅵ levels of cortex. Similarly semaphorins- 3A positive cells distributed mainly in granule cell layer of dentate gyrus, CA1, CA3 region and only a few of sema- phorins-3A positive cells scattered in the multi-line layer in hippocampus. The expression level of semaphorins-3A was significant difference among postnatal 0d,7d, 14d,21d and 28d rats （P 〈 0.01 ）. Conclusion Semaphorins- 3A positive cells widely distribute in the various cortex and hippocampus in developing rat brain ,and the region of semaphorins-3A is reduced with age growth of rats.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2012年第3期219-221,共3页
Chinese Journal of Behavioral Medicine and Brain Science
