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鱼油对开放式腹部手术大鼠术后疲劳的影响及机制 被引量:1

Effect of fish oil on postoperative fatigue of rats subject to open abdominal surgery: implications and mechanisms
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摘要 目的探讨鱼油对开放式腹部手术大鼠术后疲劳的影响及机制。方法将24只sD大鼠均分成对照组(C组)、手术组(S组)和鱼油干预组(F组)。术后观察行为学变化,检测白细胞介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH—PX)含量;光镜观察脑海马组织形态,电镜观察其超微结构;高效液相检测中枢神经递质去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)。结果病理和电镜显示F组损伤较s组轻;术后高效液相分析表明中枢不同区域的神经递质含量不同。S组血清IL-1β、IL-6、TNF—α浓度分别为(60.02±11.08)、(50.13±4.45)、(41.08±2.17)ng/L,F组为(45.48±13.51)、(42.08±4.47)、(38.76±2.41)ng/L,均较C组增高(P〈0.01),F组较S组则明显降低(P〈0.01);F组的血清SOD、GSH—PX含量较s组高(P〈0.01);F组Morris水迷宫实验指标优于S组(P〈0.05)。结论鱼油能有效改善术后的炎症反应,减轻抗氧化防御系统的损伤,减轻中枢对外周手术创伤的应激,改善术后疲劳。 Objective To investigate the effects of fish oil on postoperative fatigue (POF) and the action mechansims. Methods Twenty-four SD rats were randomly divided into control group ( group C, n = 8 ), surgery group ( group S, n = 8 ) and fish oil treatment group ( group F, n = 8). Postoperative behaviours were observed. Serum levels of interleukin (IL) -1-β, IL-6, tumor necrosis factor (TNF) -α, superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were determined. Histological changes and ultra- structural changes of neurons in the hippocampus were examined with Hematoxylin and Eosin (HE) stai- ning, and under an electron microscope. The central levels of 5-hydroxytryptamine (5-HT), dopamine (DA) and norepinephrine (NE) were measured by high-performance liquid chromatography-fluorescence. Results Histological and ultrastructural lesions in group F were milder than in group S. The contents of neurotransmitters were different. The levels of IL-1-β, IL-6 and TNF-α in group S [ (60. 02± 11.08 ), (50. 13 ±4.45), (41.08 ±2. 17) ng/L] and group F [ (45.48 ± 13.51), (42. 08 ±4.47), (38.76± 2. 41 ) ng/L] were higher than those in group C (P 〈 0.01), and those in group F were lower than in group S ( P 〈 0. 01 ) ; The levels of SOD and GSH-PX in group F were higher than in group S ( P 〈 0. 01 ). The results for Morris water maze in group F were superior to those in group S ( P 〈 0. 05 ). Conclusion Fish oil could effectively ameliorate the postoperative inflammatory reaction, reduee damage to antioxidant defense system, alleviate the eentral stress, and improve POF.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2012年第4期622-624,共3页 Chinese Journal of Experimental Surgery
基金 国家自然科学基金资助项目(81070702) 浙江省重点扶植学科项目(07-F03) 浙江省医药卫生科技计划项目(2011ZDA018)
关键词 开放式腹部手术 术后疲劳 鱼油 炎症 Open abdominal surgery Postoperative fatigue Fish oil Inflammatory
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参考文献15

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