摘要
目的探讨腺苷负荷声学造影方法定量评价不同程度缺血心肌的可行性。方法 28只健康杂种犬分为正常对照组(3只)和实验组(25只),开胸建立不同程度左前降支狭窄模型。分别实施静息、腺苷负荷心肌声学造影及氯化三苯基四氮唑、伊文思蓝染色。使用QLab高级量化软件选取ROI分析,获得曲线峰值强度(A),曲线斜率(β)和灌注量(A×β)。结果根据冠脉造影结果将实验组分为3个亚组:Ⅰ组50%～75%狭窄,Ⅱ组76%～99%狭窄,Ⅲ组100%梗阻。负荷前A值、β值和A×β值在正常对照组和实验组中逐级下降,而Ⅰ组与正常对照组间差异无统计学意义(P均>0.05);负荷后正常对照组A值、β值和A×β值均增加,Ⅰ组、Ⅱ组和Ⅲ组减少。缺血和梗死情况与标本染色一致。结论负荷声学造影可以定量评价犬缺血区心肌缺血程度。
Objective To investigate the feasibility of resting and adenosine stress real-time myocardial contrast echocardiography（MCE） on quantitative detection of myocardial perfusion abnormalities in different graded coronary stenosis of canine.Methods Twenty-eight mongrel dogs were enrolled,3of them were selected as normal group.The other 25dogs were in experiment group,and different degrees coronary stenosis was produced by constricting left anterior descending coronary artery.The resting and stressing MCE were obtained before and after coronary artery stenosis.Coronary angiography and triphenylte trazolkium chloride,Evans blue dye were then performed.Quantitative analysis was performed using QLab software on regional myocardial microbubbles filling curve,from which the A,β and A× β were got.Results Then dogs in experiment group were divided into 3subgroups,subgroup Ⅰ（50% — 75% stenosis）,Ⅱ（76% — 99% stenosis） andⅢ（100% blocking） according to coronary angiography results.At rest,A,β,A× β declined in normal and experiment group by the grade of stenosis.No difference was found between subgroupⅠ and normal group（all P〈0.05）.At stress,A,β,A× β increased in normal group while declined in subgroupⅠ,subgroupⅡandⅢ.The stained preparation reflected the ischemia and infarction regions.Conclusion MCE can quantitatively evaluate myocardial blood flow of different degrees ischemic area in canine.
出处
《中国介入影像与治疗学》
CSCD
2012年第4期286-290,共5页
Chinese Journal of Interventional Imaging and Therapy
基金
国家自然科学基金(81071158)
国家重点基础研究发展计划(973计划)项目(2011CB707900)
青年科学基金(30800271)
重庆高校优秀成果转化项目(Kjzh10201)
重庆市重点科技攻关项目(CSTC
2010AB5092)
关键词
腺苷
超声心动描记术
心肌灌注显像
冠状血管
缩窄
病理性
模型
动物
Adenosine
Echocardiography
Myocardial perfusion imaging
Coronary vessels
Constriction
pathologic
Models
animal
