摘要
选择对多菌灵、乙霉威和苯酰菌胺具有不同敏感性的胶孢炭疽菌Colletotrichum gloeosporioides、辣椒疫霉菌Phytophthora capsici及恶疫霉菌P.cactorum,采用菌丝生长速率抑制法及氨基酸序列比对法分析了其β-微管蛋白氨基酸突变与敏感性的关系。结果表明,胶孢炭疽菌对苯酰菌胺、多菌灵和乙霉威的敏感性与β-微管蛋白198位或200位氨基酸突变有关:对多菌灵敏感、对苯酰菌胺和乙霉威不敏感的胶孢炭疽菌β-微管蛋白氨基酸198位为谷氨酸(E),200位为苯丙氨酸(F);对多菌灵已产生抗性而对苯酰菌胺和乙霉威不敏感的菌株,其β-微管蛋白氨基酸200位由苯丙氨酸(F)突变为了酪氨酸(Y);对多菌灵高抗、对苯酰菌胺和乙霉威敏感的菌株其β-微管蛋白氨基酸198位由谷氨酸(E)突变为了丙氨酸(A)。辣椒疫霉菌和恶疫霉菌对苯酰菌胺敏感,对多菌灵和乙霉威均不敏感。检测疫霉菌菌株β-微管蛋白未发现氨基酸突变,但发现其β-微管蛋白氨基酸在196～200位与胶孢炭疽菌差异较大,这可能是导致苯酰菌胺仅对疫霉菌有抑制效果的原因。
The correlation between mutations of β-tubulin amino acid and the sensitivity of Colletotrichum gloeosporioides,Phytophthora capsici and P.cactorum isolates to carbendazim,diethofencarb and zoxamide was investigated by the methods of the mycelial growth inhibition and amino acid sequence alignment.The results revealed that C.gloeosporioides isolates exhibiting different sensitivity to zoxamide,carbendazim and diethofencarb were associated with the mutations in position 198 or 200 on β-tubulin amino acid sequence.Carbendazim-sensitive isolates of C.gloeosporioides with glutamic acid（E） and phenylalanine（F） at 198 and 200 sites respectively in β-tubulin amino acid sequence were insensitive to diethofencarb and zoxamide.A substitution of tyrosine（Y） for glutamic acid（F） in position 200 was detected in carbendazim-resistant but diethofencarb and zoxamide insensitive isolates.Glutamic acid（E） to alanine（A） change at position 198 was identified in carbendazim high resistant isolates that were sensitive to diethofencarb and zoxamide.All tested P.capsici and P.cactorum isolates were sensitive to zoxamide,but insensitive to carbendazim and diethofencarb.No amino acid sequence difference was detected among all Phytophthora isolates,but the sites from 196 to 200 in β-tubulin amino acid sequence of Phytophthora spp.were significantly different from that of C.gloeosporioides.These might be the reason for the sensitivity of Phytophthora spp.to zoxamide only.
出处
《农药学学报》
CAS
CSCD
北大核心
2012年第2期143-150,共8页
Chinese Journal of Pesticide Science
基金
云南省科技创新强省计划(2007AB007)
国家自然科学基金项目(30800731)
高校博士点基金(200800190025)
