摘要
目的研究慢性乙型肝炎（CHB）患者经干扰素治疗前后外周血CD4＋T细胞上可诱导共刺激分子（induciblecostimulator，ICOS）表达水平的变化情况。方法CHB患者56例，其中聚乙二醇（PEG）干扰素仪2a治疗28例，拉米夫定治疗28例，以健康志愿者20例为正常对照组。采集两治疗组治疗前及治疗后24、48周的患者外周血，以流式细胞技术检测ICOS＋CIM＋T细胞在外周血单个核细胞（peripheralbloodmononuclearceHs，PBMC）中的频数变化；采用real．timePCR法检测患者HBVDNA载量变化。结果CHB患者CD4＋T细胞ICOS表达水平明显高于正常对照者（P〈O．001）。干扰素治疗后患者ICOS表达水平下降，同拉米夫定组差异有统计学意义（P〈0．05）。干扰素治疗后患者ICOS改变值同HBVDNA载量变化值呈正相关（r=0．972，P〈O．001），而拉米夫定组则未发现相关性（r=-0．101，P=0．608）。结论CHB患者存在着细胞免疫紊乱，其CD4＋T细胞ICOS的表达升高。干扰素可下调CD4＋T细胞ICOS的表达，纠正细胞免疫偏移，发挥抗HBV作用。
Objective To study the expression levels of inducible costimulator （ICOS） on CD4＋T cells in peripheral blood in patients with chronic hepatitis B and its change after treated with interferon. Methods All 56 patients were divided into two groups （ interferon group has 28 cases, lamivudine group has 28 cases）, and interferon group treated by PEG-IFN-α 2a, lamivudine group treated by lamivudine, re- spectively. There were 20 healthy individuals as control group. The levels of ICOS on CD4＋ T cells of pa- tients with chronic hepatitis B were kinetically detected by flow cytometry. The copies of HBV DNA in sera were dynamically detected by real-time PCR. Results The levels of ICOS on CD4＋ T cells in patients with chronic hepatitis B was higher than that of normal controls （P〈0.001）. However, the expressions of ICOS on CD4＋ T cells in patients could be deduced by PEG-IFN-α 2a and obviously decreased after treatment. There was significant difference between interferon group and lamivudine group （P〈0.05 in 24 weeks, and P〈0.01 in 48 weeks）. After treatment, the change values of ICOS in interferon group was positively correla- ted with the change copies of HBV DNA （ r = 0. 972, P〈0. 001 ）. However, the change values mentioned above that did not find correlation in lamivudine group （r =-0. 101, P = 0. 608 ）. Conclusion The study shows the patients with chronic hepatitis B have disordered in cellular immunity and increased with the ex- pression of ICOS on CD4＋ T cells. PEG-IFN-α 2a could decrease the expression of ICOS on CD4＋ T cells in patients, and correct the Th2 type immune polarization to some extent, and that plays a positive role in anti- HBV.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2012年第2期124-127,共4页
Chinese Journal of Microbiology and Immunology
基金
基金项目：安徽理工大学博、硕士基金（AHLG11119）
安徽理工大学青年基金（DG752）
