期刊文献+

巴比妥共振散射光谱法测定三聚氰胺 被引量:2

Determination of melamine with barbital and resonance reyleigh scattering method
原文传递
导出
摘要 目的:建立一种共振瑞利散射方法测定食品中三聚氰胺的新方法。方法:在pH=5.5的B-R缓冲溶液中,三聚氰胺与巴比妥在四氢呋喃介质中以非共价键结合聚集形成大分子,在λ=296 nm下,激发产生强烈的共振瑞利散射。体系散射强度增强值(ΔI)与三聚氰胺浓度呈现良好的线性关系,据此建立了共振瑞利散射法测定乳制品中三聚氰胺的新方法。结果:在优化的实验条件下,浓度为3.03×10-8mol/L~1.60×10-6mol/L时,体系的共振光散射强度(ΔI)值与三聚氰胺的浓度成线性关系,线性回归方程为ΔI=1594.4c+9.70(c为三聚氰胺的浓度,10-6mol/L),相关系数r=0.9991,检出限为9.1×10-9mol/L。实验对4.0×10-8、8.0×10-8、1.2×10-7mol/L的三聚氰胺标准溶液分别进行11次平行测定,相对标准偏差分别为4.54%、3.31%、4.61%,样品加标回收率为95.0%~97.5%。结论:方法精密度、准确度好,灵敏,操作简便,用于食品中三聚氰胺的测定,结果满意。 Objective: A novel method was established for the determination of melamine with barbital and resonance rayleigh scattering(RRS).Methods: In the B-R buffer solution(pH=5.5),the melamine reacted with barbital to form large molecules by non-covalently bound in the THF medium.And a strong RRS was generated at the 296 nm.The intensity of scattering value(ΔI) was applied to determination of melamine in dairy products.Results: Under optimal conditions,the ΔI value was linear to the concentration of melamine in the range of 3.03×10-8 mol/L~1.60×10-7mol/L.And the linear regression equation was ΔI=1594.4c+9.70(c was the concentration of melamine,10-6mol/L),correlation coefficient r=0.9991,the detection limit was 9.1×10-9mol/L.When c = 4.0×10-8,8.0×10-8,1.2×10-7 mol/L,the relative standard deviations were 4.54%,3.31%,4.61%(n=11)respectively,the recoveries were 95.0%~97.5%.Conclusion: The method had advantages of good precision and accuracy,high sensitivity and simple operation,which was suitable for the determination of melamine in food.
出处 《中国卫生检验杂志》 北大核心 2012年第3期437-440,共4页 Chinese Journal of Health Laboratory Technology
基金 湖南省科技计划项目(2009FJ3067)
关键词 三聚氰胺 共振瑞利散射 巴比妥 四氢呋喃 Melamine Resonance Rayleigh Scattering Barbital Tetrahydrofuran
  • 相关文献

参考文献4

二级参考文献49

  • 1卢振举,林培滋,罗烘原,梁东白,林励吾.催化加氢转化CO_2的研究[J].环境科学,1994,15(2):59-61. 被引量:6
  • 2吴明礼,陈彩虹.高效液相色谱法(HPLC)测定单氰胺中三聚氰胺的含量[J].宁夏石油化工,2005,24(2):24-26. 被引量:44
  • 3李珊,刘忠芳,刘绍璞.胰蛋白酶与DNA相互作用的共振瑞利散射光谱及其分析应用研究[J].高等学校化学学报,2006,27(3):432-437. 被引量:17
  • 4鲁群岷,何佑秋,刘绍璞,刘忠芳.金纳米微粒作探针共振瑞利散射光谱法测定亚甲蓝[J].高等学校化学学报,2006,27(5):849-852. 被引量:24
  • 5Dobson R L M, Motlagh S, Quijano M, et al. Identification and Characterization of Toxicity of Contaminants in Pet Food Leading to an Outbreak of Renal Toxicity in Cats and Dogs [ J ]. Toxicol Sci ,2008,1011 (1) :251-262.
  • 6Roberto M V,Silvia G C M,Daniel R M,et al. Method Development and Validation for Melamine and Its Derivatives in Rice Concentrates by Liquid Chromatography Application to Animal Feed Samples [ J ]. Anal Bioanal Chem, 2008,392 (3) :523-531.
  • 7Bondar Y, Kim H J, Yoon S H,et al. Synthesis of Cation-exchange Adsorbent for Anchoring Metal Ions by Modification of Poly( glycidyl methacrylate) Chains Grafted onto Polypropylene Fabric [ J ]. React Funct Polym,2004,58( 1 ) :43-51.
  • 8Kima M,Saito K. Radiation-induced Graft Polymerization and Sulfonation of Glycidyl Methacrylate on to Porous Hollow- fiber Membranes with Different Pore Sizes [ J ]. Radiat Phys Chem ,2000 ,57 ( 2 ) : 167-172.
  • 9Kima M, Kyoichi S. Preparation of Silver-ion-loaded Nonwoven Fabric by Radiation-induced Graft Polymerization [ J ]. React Funct Polym , 1999,40 (3) :275-279.
  • 10Liang H D, Han D M, Yan X P. Cigarette Filter as Sorbent for On-line Coupling of Solid-phase Extraction to Highperformance Liquid Chromatography for Determination of Polycyclic Aromatic Hydrocarbons in Water[ J]. J Chromatogr A ,2006,11039 ( 1 ) :9-14.

共引文献154

同被引文献57

  • 1汪辉,曹小彦,彭新凯,陈利国,黄辉,谭舸.高效液相色谱-二极管阵列法测定高蛋白食品中的三聚氰胺[J].食品与机械,2007,23(5):114-115. 被引量:57
  • 2冯伟,吕昌银,严成艳,陈兴会,薛金花,程健琳.胶束增敏分光光度法水相测定乳制品中三聚氰胺[J].分析试验室,2010,29(4):49-52. 被引量:4
  • 3GB/T22388-2008,原料乳与乳制品中三聚氰胺检测方法[S].2008:2-12.
  • 4国家粮食局科学研究院.GB/T5511-2008谷物和豆类氮含量测定和粗蛋白质含量计算凯氏法[S].
  • 5GB5009.5—2010,食品安全国家标准食品中蛋白质的测定[s].
  • 6侯艳丽,张惠茹,刘来亭,兰尊海.三聚氰胺的检测方法[J].河南畜牧兽医,2007,28(09S):32-33. 被引量:46
  • 7中华人民共和国化学工业部.GB/T9567-1997工业三聚氰胺[S].
  • 8NY/T1372-2007.饲料中三聚氰胺的测定[S].
  • 9ISO 8968-1/IDF 20-1:2001 Part l:Kjelclahl method[S].
  • 10US Food and Drug Administration. FDA issues interim safety and risk assessment of melamine and melamine- related compounds in food. Approved Date: Jul. 28,2009.

引证文献2

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部